Automated free-solution isotachophoresis: instrumentation and fractionation of human serum proteins

Electrophoresis
A BöttcherG Schmitz

Abstract

An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 microm inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.

References

Jul 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K HannigK Spiegel
Mar 3, 1976·Journal of Chromatography·A KopwillemM Bier
Sep 13, 1991·Journal of Chromatography·V Kasicka, Z Prusík
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 14, 1985·Journal of Chromatography·P BocekF Foret
Jan 1, 1994·Electrophoresis·W ThormannA Schmutz
Jan 1, 1996·Methods in Enzymology·L KrivánkováP Bocek
Feb 12, 1998·Electrophoresis·G SchmitzV Richter

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