Automated, high-resolution cellular retention and uptake studies in vitro

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
Henrik Björke, Karl Andersson

Abstract

This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.

References

Feb 1, 1991·International Journal of Cancer. Journal International Du Cancer·A AnderssonB Westermark
Dec 1, 1990·Journal of Neuro-oncology·J CapalaJ Carlsson
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·P OlssonJ Carlsson
Mar 22, 2003·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Jörgen CarlssonJan Tennvall
Dec 4, 2003·Acta Oncologica·Jörgen CarlssonUNKNOWN Swedish Cancer Society Investigation Group
Aug 6, 2004·Journal of Pharmaceutical Sciences·Lisa TangBernd Meibohm
Mar 15, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Erika NordbergBengt Glimelius
Jun 14, 2005·Journal of Molecular Recognition : JMR·Annica Onell, Karl Andersson
Jul 8, 2005·Cancer Chemotherapy and Pharmacology·David J AdamsMansukh C Wani
Aug 2, 2005·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Henrik Björke, Karl Andersson

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Citations

Jun 15, 2010·Nucleosides, Nucleotides & Nucleic Acids·X Wang, F Albertioni
Feb 4, 2014·Mediators of Inflammation·Kinga BorzęckaKatarzyna Kwiatkowska
May 8, 2014·Thyroid : Official Journal of the American Thyroid Association·Filippo GalliAlberto Signore
Sep 3, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Gaurav MalviyaAlberto Signore
Jan 30, 2013·Nuclear Medicine and Biology·Hadis HonarvarPeter Järver
Oct 16, 2012·Biochemical and Biophysical Research Communications·Danièle AltschuhKarl Andersson
Apr 14, 2012·Nuclear Medicine and Biology·Zbynek NovyFrantisek Trejtnar
Sep 11, 2010·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Lars GeddaKarl Andersson
Jun 6, 2009·Biotechnology and Applied Biochemistry·Mikaela FriedmanStefan Ståhl
Apr 29, 2008·Journal of Molecular Recognition : JMR·Marika NestorHans Lundqvist
Jan 5, 2011·Journal of Molecular Recognition : JMR·Hanna BjörkelundKarl Andersson
Nov 12, 2013·Biochemical and Biophysical Research Communications·Bo XuHanna Björkelund
Aug 2, 2014·Journal of Pharmacological and Toxicological Methods·Pavel BartaKarl Andersson
Mar 12, 2008·BioTechniques·Lina VennströmKarl Andersson
May 13, 2017·International Journal of Oncology·Filippo GalliAlberto Signore
Apr 9, 2014·Nuclear Medicine Communications·Ellen WangKarl Andersson
Feb 16, 2018·Scientific Reports·Hadis HonarvarStefania De Luca
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·O L GalkinaV G Kessler
Jul 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Livia Elena ChilugGina Manda
Mar 17, 2021·Scientific Reports·Victor LehotAlain Wagner
Apr 4, 2021·International Journal of Molecular Sciences·Maryam OroujeniVladimir Tolmachev
Mar 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Gulaim A SeisenbaevaVadim G Kessler
May 1, 2021·Journal of Clinical Medicine·Filippo GalliAlberto Signore
Aug 15, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Filippo GalliAlberto Signore
Jul 2, 2016·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Zhen LiuKuangyu Shi
Jan 11, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Vladimir TolmachevAnna Orlova
Jun 2, 2021·PloS One·Sara SommarivaMichele Piana

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