Automated high-throughput infusion ESI-MS with direct coupling to a microtiter plate

Analytical Chemistry
C FeltenB L Karger

Abstract

This paper describes the design and application of instrumentation for automated high-throughput infusion ESI-mass spectrometry. The approach, based on a subatmospheric ESI interface, allows sample introduction from a commercially available microtiter plate without the need for a separate fluid delivery system. The microtiter plate was placed vertically on a three-dimensional translation stage in front of the sampling ESI interface. A single, 7-cm, 20-microm-i.d. fused-silica capillary (total volume, 70 nL), with a tapered tip, served as a combination of sample delivery and spraying capillary. The tapered tip of the capillary was enclosed in a subatmospheric chamber attached in front of the orifice of the mass spectrometer. The sample aspiration rate (flow rate) was regulated by computer-controlled pneumatic valves, which allowed fast switching of the pressure in the subatmospheric ESI chamber. A flow-through wash device was positioned between the microtiter plate and the ESI interface. This design allowed alternate filling of the capillary with (a) sample from the wells and (b) wash solution from the wash device. Sample turnaround times of 10 s/sample, with a 120-nL sample consumption/analysis, and a duty cycle (percentage of ...Continue Reading

References

Jan 5, 1999·Bioorganic & Medicinal Chemistry Letters·L SchultzJ Bukowski
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Citations

Feb 6, 2002·Current Opinion in Chemical Biology·Stephane Mouradian
Jul 26, 2011·Journal of the American Chemical Society·Leo A JoyceEric V Anslyn
Nov 27, 2004·Electrophoresis·Vladislav Dolník
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Jul 2, 2002·Analytical Chemistry·Xueheng Cheng, Jill Hochlowski
Jan 31, 2006·Journal of the American Society for Mass Spectrometry·Michael D HoffmanJuergen Kast

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