PMID: 9551604Apr 29, 1998Paper

Automated image analysis system for detecting boundaries of live prostate cancer cells

Cytometry
I SimonW A Christens-Barry

Abstract

Image analysis provides a powerful tool for quantifying cell motility and has been used to correlate motility with metastatic potential in an animal model of prostate cancer. However, widespread use of this image analysis method has been limited because earlier methods of quantitative analysis required time-intensive and subjective manual tracing of cell contours. In this report, we describe a fully automated image segmentation algorithm for detection and morphometric description of prostatic cells. The segmentation system was tested on prostate cell images generated from Hoffman modulation contrast microscopy (47 cells at 64 time points = 3,008 images) and differential interference contrast microscopy (29 cells at 64 times points plus 1 cell at 62 time points = 1,918 images). Morphometric measurements were derived from computer-determined cell boundaries and compared with the same measurements derived from manually traced cell boundaries. Final correlation coefficients for area and perimeter measurements for Hoffman and differential interference contrast microscopy were (0.76, 0.62) and (0.93, 0.93), respectively. Results with our differential interference contrast images demonstrate that our segmentation algorithm reliably an...Continue Reading

Citations

Jul 11, 2000·Journal of the American Medical Informatics Association : JAMIA·M E MattieP L Miller
Apr 27, 2013·BMC Bioinformatics·Boguslaw ObaraVicente Grau
Dec 31, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joakim LindbladAlla Zaltsman
Jan 22, 2005·Clinical and Experimental Pharmacology & Physiology·Claire L CurlLea M D Delbridge
Feb 8, 2007·Cytometry. Part B, Clinical Cytometry·Reza FarjamReza A Zoroofi
Feb 5, 2008·IEEE Transactions on Systems, Man, and Cybernetics. Part B, Cybernetics : a Publication of the IEEE Systems, Man, and Cybernetics Society·Tianzi Jiang, Faguo Yang
Aug 23, 2001·Journal of Biomedical Informatics·F Yang, T Jiang
Apr 26, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mathias GiraultKenji Yasuda
Jul 18, 2016·Medical Image Analysis·Anant Madabhushi, George Lee
Apr 25, 2006·Analytical and Bioanalytical Chemistry·Hung-Wing LiEdward S Yeung
Feb 11, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mohammad Shifat-E-RabbiGustavo K Rohde
Aug 11, 2019·Nature Reviews. Clinical Oncology·Kaustav BeraAnant Madabhushi

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