Automated integrative high-throughput phenotyping of plant shoots: a case study of the cold-tolerance of pea (Pisum sativum L.)

Plant Methods
Jan F HumplíkLukáš Spíchal

Abstract

Recently emerging approaches to high-throughput plant phenotyping have discovered their importance as tools in unravelling the complex questions of plant growth, development and response to the environment, both in basic and applied science. High-throughput methods have been also used to study plant responses to various types of biotic and abiotic stresses (drought, heat, salinity, nutrient-starving, UV light) but only rarely to cold tolerance. We present here an experimental procedure of integrative high-throughput in-house phenotyping of plant shoots employing automated simultaneous analyses of shoot biomass and photosystem II efficiency to study the cold tolerance of pea (Pisum sativum L.). For this purpose, we developed new software for automatic RGB image analysis, evaluated various parameters of chlorophyll fluorescence obtained from kinetic chlorophyll fluorescence imaging, and performed an experiment in which the growth and photosynthetic activity of two different pea cultivars were followed during cold acclimation. The data obtained from the automated RGB imaging were validated through correlation of pixel based shoot area with measurement of the shoot fresh weight. Further, data obtained from automated chlorophyll flu...Continue Reading

References

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Citations

Aug 1, 2016·Trends in Plant Science·UNKNOWN R4P Network. Electronic address: contact-r4p@listes.inra.fr
Dec 19, 2017·Frontiers in Plant Science·Zoé Joly-LopezThomas E Bureau
Jun 1, 2018·BioMed Research International·Rita ArmonienéAakash Chawade
Mar 12, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Aadil Yousuf TantrayAltaf Ahmad
Sep 26, 2020·Plant Methods·Annamalai AnandanJauhar Ali
Mar 1, 2017·Frontiers in Plant Science·Juan de la Cruz JiménezIdupulapati M Rao
Jan 10, 2018·Plant, Cell & Environment·Mostafa AbdelrahmanLam-Son Phan Tran
Apr 5, 2018·Frontiers in Plant Science·Hanne E R DenaeghelMarie-Christine Van Labeke
Oct 21, 2020·Journal of Experimental Botany·Rhonda C MeyerThomas Altmann
Jan 13, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Arun K PandeyPei Xu
Oct 24, 2018·Plant Science : an International Journal of Experimental Plant Biology·Sunil Kumar Kenchanmane RajuRebecca L Roston
May 18, 2021·Frontiers in Plant Science·Lilian P Matallana-RamirezKitt G Payn
Jul 1, 2021·Plant, Cell & Environment·Nícolas Zendonadi Dos SantosOnno Muller

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Methods Mentioned

BETA
fluorescence imaging
PlantScreen

Software Mentioned

CFIM
CFIN

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