Automated large-scale purification of a recombinant g-protein-coupled neurotensin receptor

Current Protocols in Protein Science
Jim F White, Reinhard Grisshammer

Abstract

Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance (NMR) studies, require milligram quantities of purified, functional receptor protein on a regular basis. This unit presents a step-by-step procedure for the automated two-column purification at the 10-milligram scale of a G protein-coupled receptor for neurotensin, expressed in functional form in Escherichia coli.

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Oct 5, 2011·Pharmacological Reviews·John A SalonKrzysztof Palczewski
Jan 30, 2013·Biochemical Society Transactions·Robert M CookeFiona H Marshall
Oct 17, 2012·Médecine sciences : M/S·Jean-Louis Banères, Bernard Mouillac

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