Automated linkage of proteins and payloads producing monodisperse conjugates.

Chemical Science
Igor DovganAlain Wagner

Abstract

Controlled protein functionalization holds great promise for a wide variety of applications. However, despite intensive research, the stoichiometry of the functionalization reaction remains difficult to control due to the inherent stochasticity of the conjugation process. Classical approaches that exploit peculiar structural features of specific protein substrates, or introduce reactive handles via mutagenesis, are by essence limited in scope or require substantial protein reengineering. We herein present equimolar native chemical tagging (ENACT), which precisely controls the stoichiometry of inherently random conjugation reactions by combining iterative low-conversion chemical modification, process automation, and bioorthogonal trans-tagging. We discuss the broad applicability of this conjugation process to a variety of protein substrates and payloads.

References

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Jul 25, 2019·Bioconjugate Chemistry·Igor DovganAlain Wagner

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Citations

Nov 26, 2020·Chemical Reviews·Karine PorteFrédéric Taran
May 29, 2020·Chemical Communications : Chem Comm·Margaux RiometFrédéric Taran
Aug 21, 2020·ChemistryOpen·Youshan Li, Hua Fu
Mar 11, 2020·Organic Letters·Margaux RiometFrédéric Taran

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Methods Mentioned

BETA
super-resolution microscopy
affinity purification
gel filtration

Software Mentioned

Linux
GNU
ENACT

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