Automated liquid chromatographic determination of the 20-dihydro isomers of cortisol and cortisone in human urine

Journal of Chromatography
M SchöneshöferB Weber

Abstract

A fully automated method for the simultaneous assessment of cortisol, cortisone and their 20-dihydro isomers in human urine is described. On-line sample enrichment, prepurification, focusing and injection are combined with automated high-performance liquid chromatographic separation and quantification. Losses of steroids throughout the total procedure are negligible. Thus, external calibration is feasible for quantification. Coefficients of variation range between 8.7 and 17.0% for inter-assay variability and between 1.3 and 5.2% for intra-assay variability. Assay sensitivity is 15 nmol/l. In normal students, the medians of the relative excretion rates of free 20 alpha-dihydrocortisol, 20 alpha-dihydrocortisone, 20 beta-dihydrocortisol and 20 beta-dihydrocortisone were 10.9, 6.1, 7.7 and 4.4 mumol/mol creatinine. The fully automated feature renders the present method well suited for routine diagnosis of hypercorticoidism.

References

Mar 1, 1979·The Journal of Clinical Endocrinology and Metabolism·E VocciaM I New
Oct 1, 1978·Journal of Steroid Biochemistry·B K Park
Jul 1, 1981·The Journal of Clinical Endocrinology and Metabolism·B E MurphyS C Ngo

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Citations

Apr 1, 1989·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·U TielM Schöneshöfer
Jul 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·P HeilmannM Schöneshöfer
Jun 1, 1987·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·B EisenschmidM Schöneshöfer
Apr 22, 2020·Molecular and Cellular Endocrinology·Janina TokarzJerzy Adamski
May 5, 1989·Journal of Chromatography·M SchöneshöferW Schwartzkopff
Jul 29, 1988·Journal of Chromatography·P M Kabra
Jul 3, 1987·Journal of Chromatography·M SchöneshöferR Rejaibi
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·P Volin

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