Automated method for determination of oxygen equilibrium curves of red cell suspensions under controlled buffer conditions and its clinical applications

Critical Care Medicine
T Asakura

Abstract

The accurate determination of the oxygen equilibrium curve (OEC) of whole blood or red cell suspensions requires special considerations to avoid the secondary effects due to lactate formation, removal of carbon dioxide, use of anticoagulants, etc. An automated apparatus has been constructed that can record the whole OEC of red cells within 20 min. The instrument also can be used to record the OEC of hemolysate. The pH, temperature, and PO2 are monitored constantly during the measurement. Using this instrument, the effects of pH, temperature, carbon dioxide, anticoagulants, and buffers on the OEC of normal fresh blood have been investigated. The OEC of normal blood, blood containing abnormal hemoglobin, or enzyme, were determined. The results were compared with those obtained using commercial OEC apparatuses.

References

Sep 10, 1982·European Journal of Pharmacology·T Sato, S T Ohnishi
Aug 1, 1991·Respiration Physiology·D BöningS Göke
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·P Vorger, D Matelin
Dec 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·C F Cartheuser
Jul 9, 2002·British Journal of Haematology·Efemwonkiekie W IyamuT Asakura
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T AsakuraE Schwartz
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·T AsakuraK Ohene-Frempong
Oct 21, 2004·American Journal of Hematology·Osheiza AbdulmalikT Asakura
Oct 16, 2015·Blood Cells, Molecules & Diseases·Siddharth ShahT Asakura
Sep 28, 2010·American Journal of Hematology·Osheiza AbdulmalikJ Eric Russell
Feb 15, 1984·Archives of Biochemistry and Biophysics·K KikugawaT Asakura
Jan 1, 1989·American Journal of Medical Genetics·A VillegasD Espinós
Nov 24, 2004·Biochemical and Biophysical Research Communications·Zhenning He, J Eric Russell

Related Concepts

Buffers
Autoanalysis Procedure
Dioxygen
Whole Blood
Lactate Biosynthetic Process
Ampholytes
Whole Blood Specimen
Abnormal Hemoglobins
Oxyhemoglobin
Carbon Dioxide

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.