Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Kathrin GassmannEllen Fritsche

Abstract

Existing guidelines for testing developmental neurotoxicity (DNT) propose investigations in rodents, which are ethically questionable as well as time and cost intensive. Thus, there is international agreement that predictive in vitro methods are needed to increase efficiency of testing and limit the number of animals used. One of a variety of novel approaches for DNT testing utilizes neurospheres, three-dimensional aggregate cultures of primary normal neural progenitor cells (NPCs). Because sorting and plating of single neurospheres is one of the most time-consuming steps within the assay, the aim of this study was to evaluate if the complex object parametric analyzer and sorter (COPAS PLUS(TM), Union Biometrica Inc.) is a suitable tool for automated sorting and plating of neurospheres. The results of the comparison of NPC viability, proliferation, migration, differentiation and intracellular oxidative stress between manually and COPAS sorted and plated neurospheres of different species show that the automation by the COPAS instrument does not influence the basic performance of neurospheres. Therefore, we consider the COPAS instrument as a useful tool for higher throughput neurosphere research in toxicology, neuroregeneration, ...Continue Reading

References

Jan 5, 1999·Journal of Neuroscience Methods·C N SvendsenM A Caldwell
Jul 26, 2003·Cancer Letters·Dirk GeertsRogier Versteeg
Sep 6, 2003·Free Radical Biology & Medicine·Toby N Behar, Carol A Colton
Feb 10, 2006·American Journal of Physiology. Renal Physiology·R Lance MillerRaoul D Nelson
Dec 19, 2006·Lancet·P Grandjean, P J Landrigan
Feb 20, 2007·Molecular Neurobiology·Josephine B Jensen, Malin Parmar
Apr 21, 2007·Toxicology and Applied Pharmacology·Michaela MoorsEllen Fritsche
Aug 28, 2007·Cell·Kenneth M Yamada, Edna Cukierman
Sep 2, 2008·Brain Research·Rajesh K SharmaPeter A Netland
Dec 17, 2008·Risk Analysis : an Official Publication of the Society for Risk Analysis·Robert J KavlockRaymond R Tice
Feb 4, 2009·Antioxidants & Redox Signaling·Hanke L MatlungEd VanBavel
Jun 30, 2009·Neurotoxicology and Teratology·Joseph M BreierTimothy J Shafer
Aug 6, 2009·Environmental Health Perspectives·Michaela MoorsEllen Fritsche
May 21, 2010·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M IngramC R Taylor
Sep 8, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Alice A ChenSangeeta N Bhatia

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Citations

Nov 19, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Martje W G D M de GrootRemco H S Westerink
Jul 6, 2013·Journal of Bioscience and Bioengineering·Hideki Mori, Masayuki Hara
Oct 18, 2015·Journal of Bioscience and Bioengineering·Hideki Mori, Masayuki Hara
Apr 16, 2015·Future Medicinal Chemistry·Shane R HormanChristophe Antczak
Jun 11, 2014·Experimental Biology and Medicine·David PamiesHelena T Hogberg
Jun 6, 2014·Current Protocols in Toxicology·Jenny BaumannEllen Fritsche
Jan 28, 2020·Recent Patents on Drug Delivery & Formulation·Anmol DograJasjeet Kaur Narang
Jun 6, 2014·Alternatives to Laboratory Animals : ATLA·Costanza RovidaJürgen Hescheler

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