Automated online coupling of robot-assisted single drop microextraction and liquid chromatography

Journal of Chromatography. a
Deyber Arley Vargas MedinaÁlvaro José Santos-Neto

Abstract

A high-throughput and innovative setup has been developed to automate the online integration of single drop microextraction (SDME), liquid chromatography (LC) and high-resolution mass spectrometry (QqToF). SDME and LC were online hyphenated for the first time. SDME was carried out by a lab-made cartesian robot actuating a 100 μL syringe, equipped with a three-way solenoid microvalve that allowed the online transference of the enriched extract to the chromatographic system, through a six-port switching valve. The complete method, including the synchronized robot action, valves, and the analytical instruments, was controlled by an Arduino Mega board. The merits of the proposed setup were demonstrated by the triazines determination in coconut water samples. The most relevant extraction parameters, such as drop size, exposure time, stirring effect, salt addition and pH were systematically investigated. Under optimized conditions (60 μL drop volume and 10 min extraction time), the LC-UV enrichment factors (EF) and the extraction recoveries (ER) ranged between 15.2-18.4 and 11.4-13.8%, respectively. Using the SDME-LC-MS setup, the linear range, detection limit (S/N = 3) and precision (RSD, n = 6 at 0.25 μg L-1 level of concentration)...Continue Reading

Citations

Aug 8, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Chao LiJinkai Xue
Nov 17, 2019·Analytical and Bioanalytical Chemistry·Luis Felipe Rodríguez CabalÁlvaro José Santos-Neto
Aug 20, 2019·Journal of Chromatography. a·Luis Felipe Rodríguez CabalÁlvaro Jose Santos-Neto
Oct 19, 2019·Analytical Chemistry·Frederik A Hansen, Stig Pedersen-Bjergaard
Aug 14, 2020·Chemical Reviews·Gurpur Rakesh D Prabhu, Pawel L Urban
Dec 18, 2021·Journal of Separation Science·Guizhen Li, Kyung Ho Row

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