PMID: 18187866Jan 12, 2008Paper

Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Koshi ImamiYasushi Ishihama

Abstract

We have developed an on-line automated system for phosphoproteome analysis using titania-based phosphopeptide enrichment followed by nanoLC-MS/MS. Titania beads were prepared by calcination of commercial chromatographic titania beads at 800 degrees C to convert the crystalline structure. The obtained rutile-form titania exhibited higher selectivity in phosphopeptide enrichment than commercial titania, even in the absence of a competitive chelating reagent for non-phosphopeptides. For phosphoproteome analysis of human cervical cancer HeLa cells, tryptic digests of the cell extracts were directly injected into this on-line system, and 696 non-redundant phosphopeptides with 671 unambiguously determined phosphorylation sites, derived from 512 phosphoproteins, were successfully identified. This is the first successful application of an on-line automated phosphoproteome analysis system to complex biological samples.

References

Jan 27, 2000·Cell·T Hunter
Aug 19, 2000·Journal of Chromatography. a·J Winkler, S Marmé
May 18, 2001·Nature·P Blume-Jensen, T Hunter
Mar 5, 2002·Nature Biotechnology·Scott B FicarroForest M White
Dec 25, 2002·Journal of Chromatography. a·Yasushi IshihamaMatthias Mann
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Apr 8, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Akira Sano, Hiroshi Nakamura
Oct 14, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ikuma KurodaMasahiro Furuno
Jan 25, 2005·Molecular & Cellular Proteomics : MCP·Albrecht GruhlerOle N Jensen
Apr 29, 2005·Molecular & Cellular Proteomics : MCP·Martin R LarsenThomas J D Jørgensen
Oct 27, 2005·Molecular & Cellular Proteomics : MCP·Jesper V OlsenMatthias Mann
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann
Feb 16, 2006·Analytical Biochemistry·Koji HataMitsuyoshi Ueda
Mar 16, 2006·Analytical Chemistry·Hye Kyong Kweon, Kristina Håkansson
Oct 7, 2006·Journal of Proteome Research·Yasmine M NdassaMark M Ross
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Judit VillénSteven P Gygi
Nov 10, 2011·Science Signaling·Björn HegemannJan-Michael Peters
Feb 9, 2012·Molecular & Cellular Proteomics : MCP·Angus I LamondMatthias Mann

❮ Previous
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Citations

Mar 12, 2013·Chromosoma·Dan N Simon, Katherine L Wilson
Oct 18, 2011·Amino Acids·Jan Fíla, David Honys
Apr 12, 2011·Cell Biochemistry and Biophysics·Benedikt M Kessler, Mariola J Edelmann
Feb 24, 2010·The Journal of Biological Chemistry·Claudia PerreraLuisa Rusconi
Jan 13, 2009·Molecular & Cellular Proteomics : MCP·Nozomu YachieYasushi Ishihama
Jan 13, 2010·Journal of Biochemistry·Yuka IwaseTsuneyoshi Horigome
Aug 5, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jennifer D CohenSerrine S Lau
May 23, 2013·Science Signaling·Tobias LangenhanJörg Hamann
Aug 14, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Raul Covian, Robert S Balaban
Feb 18, 2011·Endocrinology·Paul LanglaisLawrence J Mandarino
Feb 10, 2011·Journal of Cell Science·Katie R MartinJeffrey P MacKeigan
Mar 18, 2009·The Journal of Cell Biology·Praveen KumarTorsten Wittmann
Dec 19, 2015·Journal of Proteome Research·Fei LiuMark S Baker
Jun 9, 2012·Seminars in Cell & Developmental Biology·Kristoffer T G Rigbolt, Blagoy Blagoev
Jun 12, 2012·Seminars in Cell & Developmental Biology·Haruna ImamuraYasushi Ishihama
Mar 9, 2010·Biochemical and Biophysical Research Communications·Eric A Gustafson, Gary M Wessel
Oct 23, 2010·Glia·Keiichi YasudaMasami Takahashi
Apr 15, 2011·British Journal of Pharmacology·Stephan KempRonald J A Wanders
Oct 25, 2013·The FEBS Journal·Mark P Thomas, Barry V L Potter
Nov 15, 2013·Proteomics·Yoko InoHisashi Hirano
Jan 17, 2012·The Biochemical Journal·Mark A BarberHeidi C E Welch
Oct 25, 2008·Biochemical and Biophysical Research Communications·Karen D CorbinDuane C Eichler
Oct 5, 2010·Trends in Biochemical Sciences·Victor M Bolanos-Garcia, Tom L Blundell
Feb 26, 2016·Journal of the American Society for Mass Spectrometry·Wenjie LiuHerbert H Hill
Dec 30, 2014·Journal of Chromatography. a·Xiao-Shui LiYu-Qi Feng
Oct 30, 2018·Current Protein & Peptide Science·Mónica Rodríguez-Bolaños, Ruy Perez-Montfort
Mar 12, 2010·The Journal of Biological Chemistry·Sheena D'ArcyVictor M Bolanos-Garcia
Oct 30, 2009·The Journal of Biological Chemistry·Kathryn G FosterDiane C Fingar

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