Automated point-of-care testing for ABO agglutination test: proof of concept and validation

Vox Sanguinis
H El Kenz, F Corazza

Abstract

ABO-incompatible red blood cell transfusions still represent an important hazard in transfusion medicine. Therefore, some countries have introduced a systematic bedside ABO agglutination test checking that the right blood is given to the right patient. However, this strategy requires an extremely time-consuming learning programme and relies on a subjective interpretation of ABO test cards agglutination. We developed a prototype of a fully automated device performing the bedside agglutination test that could be completed by reading of a barcoded wristband. This POCT checks the ABO compatibility between the patient and the blood bag. Proof of concept and analytical validation of the prototype has been completed on 451 blood samples: 238 donor packed red blood cells, 137 consecutive unselected patients for whom a blood group determination had been ordered and on 76 patient samples selected with pathology that could possibly interfere with or impair performances of the assay. We observed 100% concordance for ABO blood groups between the POCT and the laboratory instrument. These preliminary results demonstrate the feasibility of ABO determination with a simple POCT device eliminating manipulation and subjective interpretation respon...Continue Reading

References

Dec 6, 2005·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·D Stainsby
Aug 16, 2006·Vox Sanguinis·P Pagliaro, P Rebulla
Nov 10, 2006·British Journal of Haematology·Walter H Dzik
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Aug 13, 2010·Clinical Chemistry·Marion L SnyderCorinne R Fantz
Sep 17, 2013·British Journal of Haematology·Paula H B Bolton-Maggs, Hannah Cohen

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Citations

Feb 7, 2018·Journal of Laboratory Physicians·Aseem Kumar TiwariDevi Prasad Acharya
Jun 10, 2021·Clinical Microbiology Reviews·Nancy E CornishSheldon Campbell

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