Automated Processing and Evaluation of Anti-Nuclear Antibody Indirect Immunofluorescence Testing

Frontiers in Immunology
Vincent RicchiutiJames K Fleming

Abstract

Indirect immunofluorescence (IIF) is considered by the American College of Rheumatology (ACR) and the international consensus on ANA patterns (ICAP) the gold standard for the screening of anti-nuclear antibodies (ANA). As conventional IIF is labor intensive, time-consuming, subjective, and poorly standardized, there have been ongoing efforts to improve the standardization of reagents and to develop automated platforms for assay incubation, microscopy, and evaluation. In this study, the workflow and performance characteristics of a fully automated ANA IIF system (Sprinter XL, EUROPattern Suite, IFA 40: HEp-20-10 cells) were compared to a manual approach using visual microscopy with a filter device for single-well titration and to technologist reading. The Sprinter/EUROPattern system enabled the processing of large daily workload cohorts in less than 8 h and the reduction of labor hands-on time by more than 4 h. Regarding the discrimination of positive from negative samples, the overall agreement of the EUROPattern software with technologist reading was higher (95.6%) than when compared to the current method (89.4%). Moreover, the software was consistent with technologist reading in 80.6-97.5% of patterns and 71.0-93.8% of titers...Continue Reading

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Citations

May 2, 2021·Journal of Autoimmunity·Maud WilhelmSylviane Muller

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Methods Mentioned

BETA
ELISA

Software Mentioned

EUROpattern
GraphPad
Helios
LabCorp
Image Navigator
Zenit G - Sight
- Pattern Suite
AKLIDES
EURO
GraphPadPrism

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