Automated sample preparation for cholesterol determination in foods

Journal of Chromatography. a
J H JohnsonJ Zdunek

Abstract

An automated sample preparation system has been developed for the determination of cholesterol in a wide range of matrices. Isolation of cholesterol is performed with a robotic arm coupled with a series of modular stations. Samples are introduced into the system which adds the appropriate reagents, carries out the saponification, pH adjustment, solid-phase extraction and drying steps. This system was evaluated using 15 different food matrices. The average recovery for NIST standards exceeded 97%. A solution of n-hexane-2-propanol was substituted for the traditional methanol-chloroform extraction. Manual pH adjustment was replaced with a buffer. Manual and automated methods were compared and no difference was observed at the 95% confidence level.

References

Oct 30, 1992·Journal of Chromatography·M Fenton
Oct 1, 1983·Journal of Dairy Science·W J HurstR A Martin
Feb 23, 1982·Biochimica Et Biophysica Acta·P OttU Brodbeck

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Citations

Jun 16, 2010·Analytical and Bioanalytical Chemistry·Bogusław BuszewskiMichał Szumski
Aug 15, 2000·Journal of Chromatography. a·D T Rossi, N Zhang
Aug 15, 2000·Journal of Chromatography. a·V Ruiz-Gutiérrez, M C Pérez-Camino

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