PMID: 8947534Nov 1, 1996Paper

Automated scoring of micronuclei in binucleated human lymphocytes

International Journal of Radiation Biology
W BöckerC Yu

Abstract

Manual and automatic scoring of micronuclei (MN) in binucleated human lymphocytes (BNC) were compared after irradiation of whole blood samples. The blood samples were irradiated with X-ray doses (1, 2 or 3 Gy) and stained with Giemsa. The preparation technique was optimized in such a way that acceptable conditions (cell density, contrast) were obtained for both scoring procedures. To estimate the quality of automatic micronucleus detection, two researchers who had different experience in scoring MN (6 months and 5 years) analysed the samples independently from each other. Automatic scoring was carried out with a digital image analysis system and the recognition procedure was divided into two parts. The BNC positions were detected with low microscope magnification (100x), and the recognition of micronuclei within the cytoplasm of the classified BNC was carried out at high magnification (630x). A fuzzy logic classification system as well as two different segmentation steps (preclassification and postclassification) made it possible that about 94% of all automatically recognized BNC were classified correctly). On the other hand, the classification system was optimized in such a way that false positive decisions were minimized (95%...Continue Reading

References

Dec 1, 1992·International Journal of Radiation Biology·G A SchreiberM Nüsse
Nov 1, 1990·International Journal of Radiation Biology·A D TatesJ S Ploem
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
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Citations

Dec 18, 2010·Mutagenesis·Zbigniew DarzynkiewiczMichael Fenech
Jan 31, 2016·Journal of Radiation Research·Ayumi NakamuraYasushi Mariya
Dec 2, 2008·Mutation Research·Ana María SalazarPatricia Ostrosky-Wegman
Apr 21, 2007·Mutation Research·Dolores DiazChester Costales
Nov 2, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Lei ChengAndrzej Wojcik
Oct 22, 2010·Environmental and Molecular Mutagenesis·Magdalena LukamowiczAzeddine Elhajouji
Jul 24, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Xue-Kun YanPing-Kun Zhou
Jan 18, 2006·International Journal of Radiation Oncology, Biology, Physics·Yuhchyau ChenBranislav Jeremic
Dec 30, 2014·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·G Tamizh SelvanP Venkatachalam
Jun 6, 1998·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·K WuttkeC Streffer
Oct 13, 2001·Cytometry·P SmolewskiZ Darzynkiewicz

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