Automated typing of red blood cell and platelet antigens: a whole-genome sequencing study

The Lancet. Haematology
William J LaneMedSeq Project

Abstract

There are more than 300 known red blood cell (RBC) antigens and 33 platelet antigens that differ between individuals. Sensitisation to antigens is a serious complication that can occur in prenatal medicine and after blood transfusion, particularly for patients who require multiple transfusions. Although pre-transfusion compatibility testing largely relies on serological methods, reagents are not available for many antigens. Methods based on single-nucleotide polymorphism (SNP) arrays have been used, but typing for ABO and Rh-the most important blood groups-cannot be done with SNP typing alone. We aimed to develop a novel method based on whole-genome sequencing to identify RBC and platelet antigens. This whole-genome sequencing study is a subanalysis of data from patients in the whole-genome sequencing arm of the MedSeq Project randomised controlled trial (NCT01736566) with no measured patient outcomes. We created a database of molecular changes in RBC and platelet antigens and developed an automated antigen-typing algorithm based on whole-genome sequencing (bloodTyper). This algorithm was iteratively improved to address cis-trans haplotype ambiguities and homologous gene alignments. Whole-genome sequencing data from 110 MedSeq ...Continue Reading

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Citations

Aug 24, 2018·Current Opinion in Hematology·Marsha M Wheeler, Jill M Johnsen
Feb 2, 2019·British Journal of Haematology·Catherine A HylandElizna M Schoeman
Jul 19, 2019·Transfusion Medicine·D J Roberts, M Delaney
Aug 9, 2019·Transfusion·William J LaneUNKNOWN MilSeq Project
Aug 15, 2019·Expert Review of Molecular Diagnostics·Carolina Bonet Bub, Lilian Castilho
Feb 28, 2019·Blood·Connie M Westhoff
Oct 10, 2019·Transfusion·Richard M Kaufman
Sep 7, 2019·Current Opinion in Hematology·Celina MontemayorMargaret A Keller
Sep 2, 2020·Transfusion·Marianne StefGorka Ochoa-Garay
Mar 13, 2019·Transfusion Medicine·T BullockJ McCullagh
Nov 13, 2020·PloS One·Julien PaganiniJulie Di Cristofaro
May 13, 2021·Transfusion Medicine Reviews·Jill R Storry
May 18, 2021·Transfusion Medicine Reviews·Aline FlochFrance Pirenne
Feb 29, 2020·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Daniel FürstChristof Weinstock
Dec 1, 2021·Transfusion Medicine·Joost H J van SambeeckMart P Janssen

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