Automated video tracking of thrips behavior to assess host-plant resistance in multiple parallel two-choice setups

Plant Methods
Manus Pm ThoenMaarten A Jongsma

Abstract

Piercing-sucking insects cause severe damage in crops. Breeding for host-plant resistance can significantly reduce the yield losses caused by these insects, but host-plant resistance is a complex trait that is difficult to phenotype quickly and reliably. Current phenotyping methods mainly focus on labor-intensive and time-consuming end-point measurements of plant fitness. Characterizing insect behavior as a proxy for host-plant resistance could be a promising time-saving alternative to end-point measurements. We present a phenotyping platform that allows screening for host-plant resistance against Western flower thrips (WFT, Frankliniella occidentalis (Pergande)) in a parallel two-choice setup using automated video tracking of thrips behavior. The platform was used to establish host-plant preference of WFT with a large plant population of 345 wild Arabidopsis accessions and the method was optimized with two extreme accessions from this population that differed in resistance towards WFT. To this end, the behavior of 88 WFT individuals was simultaneously tracked in 88 parallel two-choice arenas during 8 h. Host-plant preference of WFT was established both by the time thrips spent on either accession and various behavioral paramet...Continue Reading

References

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Jul 29, 2009·BMC Plant Biology·Hiroshi AbeMasatomo Kobayashi
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Jun 1, 2015·Current Opinion in Insect Science·Fiona L GogginChristopher N Topp

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Citations

Jan 28, 2017·Pest Management Science·Sanae MoudenKirsten A Leiss
Mar 1, 2018·Journal of Experimental Botany·Merel SteenbergenColette Broekgaarden
Nov 22, 2018·Frontiers in Plant Science·Rajagopalbabu SrinivasanSoraya C M Leal-Bertioli
Feb 15, 2019·Frontiers in Plant Science·Maarten A JongsmaJohannes W Kruisselbrink
Oct 8, 2020·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Maria T BekendamPaula M C Mommersteeg
Jan 21, 2021·Molecular Genetics and Genomics : MGG·Tristan CumerThibaut Capblancq

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Software Mentioned

Ethovision XT
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