Automated whole animal bio-imaging assay for human cancer dissemination.

PloS One
Veerander P S GhotraErik H J Danen

Abstract

A quantitative bio-imaging platform is developed for analysis of human cancer dissemination in a short-term vertebrate xenotransplantation assay. Six days after implantation of cancer cells in zebrafish embryos, automated imaging in 96 well plates coupled to image analysis algorithms quantifies spreading throughout the host. Findings in this model correlate with behavior in long-term rodent xenograft models for panels of poorly- versus highly malignant cell lines derived from breast, colorectal, and prostate cancer. In addition, cancer cells with scattered mesenchymal characteristics show higher dissemination capacity than cell types with epithelial appearance. Moreover, RNA interference establishes the metastasis-suppressor role for E-cadherin in this model. This automated quantitative whole animal bio-imaging assay can serve as a first-line in vivo screening step in the anti-cancer drug target discovery pipeline.

References

Sep 1, 1988·Clinical & Experimental Metastasis·D H ShevrinT E Lad
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jun 28, 2002·Cancer Cell·James F AmatrudaLeonard I Zon
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Jul 2, 2003·Nature Reviews. Cancer·Howard M Stern, Leonard I Zon
Feb 6, 2004·Breast Cancer Research and Treatment·Marc Lacroix, Guy Leclercq
Feb 3, 2005·Nature Reviews. Drug Discovery·Leonard I Zon, Randall T Peterson
Jun 22, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lisa M J LeeMary J C Hendrix
Aug 25, 2005·BioTechniques·Stephane BerghmansJohn P Kanki
Apr 4, 2006·Cell Cycle·Siew Hong Lam, Zhiyuan Gong
Apr 6, 2007·Cancer Research·Stefania NicoliMarco Presta
Oct 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Konstantin StoletovRichard Klemke
Dec 17, 2008·International Review of Cell and Molecular Biology·James F Amatruda, E Elizabeth Patton
Jun 10, 2010·Journal of Cell Science·Konstantin StoletovRichard Klemke
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg

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Citations

Oct 10, 2012·Future Medicinal Chemistry·Jonathan R MathiasJeff S Mumm
Dec 3, 2013·Endocrine-related Cancer·Giovanni VitaleLuca Persani
Oct 26, 2013·BMC Research Notes·Magnus PalmbladHerman P Spaink
Jul 16, 2013·Interface Focus·Fränze ProgatzkyCristina Lo Celso
Aug 24, 2013·Nature Reviews. Cancer·Richard WhiteLeonard Zon
Aug 21, 2012·Annals of the New York Academy of Sciences·Martina KonantzClaudia Lengerke
Oct 8, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Feng ZhuDonald Wlodkowic
Jul 1, 2014·The Journal of Pathology·Wietske van der EntB Ewa Snaar-Jagalska
Aug 12, 2015·Journal of Experimental & Clinical Cancer Research : CR·Shuai ZhaoJun Ye
Jan 30, 2013·Biochimica Et Biophysica Acta·Chiara TobiaMarco Presta
Apr 10, 2014·Cell Adhesion & Migration·Aviv BergmanBojana Gligorijevic
Jun 26, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Miloš VittoriTamara Lah Turnšek
Jan 30, 2018·NPJ Precision Oncology·Matteo AstoneLuke H Hoeppner
Jun 7, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Massimo M Santoro
May 3, 2019·Toxicology Mechanisms and Methods·Carlha Gutiérrez-LoveraLaura Sánchez
Jan 5, 2019·F1000Research·David HillBill Chaudhry
Jan 13, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jorge BarriusoAdam Hurlstone
Nov 13, 2014·Cancer Research·Veerander P S GhotraErik H J Danen
Oct 18, 2018·Cancer Research·Patricia LetradoJulen Oyarzabal
Mar 2, 2021·Frontiers in Cell and Developmental Biology·Xingyu ChenRenbing Jia
Apr 14, 2019·Experimental Cell Research·Na ChenNienke Vrisekoop
Jun 13, 2021·Nature Reviews. Drug Discovery·E Elizabeth PattonDavid M Langenau
Jul 3, 2021·Pharmaceuticals·Hissa F Al-ThaniHuseyin C Yalcin
Aug 31, 2019·Bulletin du cancer·Ludivine RabyPierre-Olivier Angrand

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Methods Mentioned

BETA
xenograft
FACS
xenografts
transgenic

Software Mentioned

EZ C1
Prism
Pro
GraphPad
Pro Plus
Image
excel

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