Automated whole-cell patch-clamp electrophysiology of neurons in vivo.

Nature Methods
Suhasa B KodandaramaiahCraig R Forest

Abstract

Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.

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Citations

Mar 22, 2013·Science Translational Medicine·Brian Y Chow, Edward S Boyden
Oct 5, 2013·BMC Bioinformatics·Hanchuan PengGiorgio A Ascoli
Jul 7, 2014·Nature Neuroscience·Amy S ChuongEdward S Boyden
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Mar 5, 2016·Nature Protocols·Suhasa B KodandaramaiahEdward S Boyden
Jul 12, 2013·Annals of the New York Academy of Sciences·Suhasa B KodandaramaiahCraig R Forest
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Apr 15, 2016·Nature·Helen Shen
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Software Mentioned

MATLAB
autopatcher
Photoshop
Digidata
Clampex
Clampfit
ImageJ
Labview

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