Automated 'X-Y' robot for peptide synthesis with microwave heating: application to difficult peptide sequences and protein domains

Journal of Peptide Science : an Official Publication of the European Peptide Society
Leila MalikKnud J Jensen

Abstract

Precise microwave heating has emerged as a valuable method to aid solid-phase peptide synthesis (SPPS). New methods and reliable protocols, as well as their embodiment in automated instruments, are required to fully use this potential. Here we describe a new automated robotic instrument for SPPS with microwave heating, report protocols for its reliable use and report the application to the synthesis of long sequences, including the beta-amyloid 1-42 peptide. The instrument is built around a valve-free robot originally developed for parallel peptide synthesis, where the robotic arm transports reagents instead of pumping reagents via valves. This is the first example of an 'X-Y' robotic microwave-assisted synthesizer developed for the assembly of long peptides. Although the instrument maintains its capability for parallel synthesis at room temperature, in this paper, we focus on sequential peptide synthesis with microwave heating. With this valve-free instrument and the protocols developed for its use, fast and efficient syntheses of long and difficult peptide sequences were achieved.

References

Aug 9, 2002·Nature·Rachel L BatterhamStephen R Bloom
Nov 9, 2002·Organic Letters·Hernando J OlivosThomas Kodadek
Aug 26, 2004·Protein and Peptide Letters·Anna K TicklerJohn D Wade
Feb 25, 2005·Organic Letters·Takahiko MatsushitaShin-Ichiro Nishimura
May 9, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Toni VagtBeate Koksch
Jun 22, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Bernadett BacsaC Oliver Kappe
Nov 24, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stacey A PalasekJonathan M Collins
Dec 18, 2007·The Journal of Biological Chemistry·Takayuki OhnumaEtsuko Katoh

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Citations

Sep 25, 2012·Chemical Communications : Chem Comm·Gabrielle N Thibodeaux, Wilfred A van der Donk
Aug 2, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Fabio RizzoloAnna Maria Papini
May 29, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ramon Subirós-FunosasFernando Albericio
Aug 7, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Søren L PedersenKnud J Jensen
Feb 25, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Keiko MasudaKazuyoshi Ukena

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