Apr 21, 2020

Downregulation of a Type III Secretion System under Reducing Conditions in Bordetella pertussis

BioRxiv : the Preprint Server for Biology
M. GotoAsaomi Kuwae

Abstract

Bordetella pertussis uses a type III secretion system (T3SS) to inject virulence proteins into host cells. Although the B. pertussis T3SS would play a role in host colonization, the efficient secretion of type III secreted proteins from B. pertussis has not been observed. To investigate the roles of type III secreted proteins during infection, we attempted to optimize culture conditions for the production and secretion of a type III secreted protein, BteA, in B. pertussis. We observed that B. pertussis efficiently secretes BteA in ascorbic acid-depleted (AsA-) medium. When L2 cells, a rat lung epithelial cell line, were infected with B. pertussis cultured in the AsA- medium, BteA-dependent cytotoxicity was observed. We also performed an immunofluorescence assay of L2 cells infected with B. pertussis. The clear fluorescence signals of Bsp22, a needle structure of T3SS, were detected on the bacterial surface of B. pertussis cultured in the AsA- medium. Moreover, when B. pertussis was cultured in liquid medium containing other reducing agents such as 2-mercaptoethanol and dithioerythritol, the production of type III secreted proteins was repressed. These results suggest that in B. pertussis, the production and secretion of type II...Continue Reading

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