Automatic evaluation of tumor budding in immunohistochemically stained colorectal carcinomas and correlation to clinical outcome

Diagnostic Pathology
Cleo-Aron WeisTimo Gaiser

Abstract

Tumor budding, meaning a detachment of tumor cells at the invasion front of colorectal carcinoma (CRC) into single cells or clusters (<=5 tumor cells), has been shown to correlate to an inferior clinical outcome by several independent studies. Therefore, it has been discussed as a complementary prognostic factor to the TNM staging system, and it is already included in national guidelines as an additional prognostic parameter. However, its application by manual evaluation in routine pathology is hampered due to the use of several slightly different assessment systems, a time-consuming manual counting process and a high inter-observer variability. Hence, we established and validated an automatic image processing approach to reliably quantify tumor budding in immunohistochemically (IHC) stained sections of CRC samples. This approach combines classical segmentation methods (like morphological operations) and machine learning techniques (k-means and hierarchical clustering, convolutional neural networks) to reliably detect tumor buds in colorectal carcinoma samples immunohistochemically stained for pan-cytokeratin. As a possible application, we tested it on whole-slide images as well as on tissue microarrays (TMA) from a clinically ...Continue Reading

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Citations

Dec 18, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·J M BokhorstI D Nagtegaal
Apr 12, 2020·Cancer Communications·Yahui JiangYan Sun
Sep 10, 2020·Nature Reviews. Clinical Oncology·Alessandro LugliIris D Nagtegaal
Sep 29, 2020·Computers in Biology and Medicine·Ishak PacalUfuk Nalbantoglu
Apr 26, 2021·The American Journal of Pathology·Arvydas LaurinaviciusRichard Levenson
Jun 18, 2021·World Journal of Gastroenterology : WJG·Hiroshi Yoshida, Tomoharu Kiyuna
Nov 6, 2021·The Journal of Pathology·Scarlet BrockmoellerJakob Nikolas Kather

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