Automatic gas chromatographic determination of the high-density-lipoprotein cholesterol and total cholesterol in serum

Journal of Chromatography. B, Biomedical Applications
M S CárdenasM Valcárcel

Abstract

A new analytical method that combines on-line precipitation-filtration, enzymatic hydrolysis, extraction and gas chromatography was developed for the determination of total cholesterol and high-density-lipoprotein cholesterol in human serum. Very-low-density lipoprotein, intermediate-density lipoprotein and low-density lipoprotein are precipitated with sodium phosphotungstate and magnesium chloride; then, the serum is continuously filtered and unprecipitated high-density-lipoprotein cholesterol is enzymatically hydrolyzed and finally determined as cholesterol by gas chromatography. Total cholesterol is also determined by direct introduction of the serum into the proposed system. The proposed method was validated by analyzing a lipid control serum with certified contents of high-density-lipoprotein cholesterol and total cholesterol. The results obtained were consistent with the certified contents.

References

Jul 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·J M Fernández-RomeroM Valcárcel
Aug 31, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K A Kovar, F El-Yazbi
Jan 1, 1982·The Journal of Automatic Chemistry·C G Fraser

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Citations

Jan 5, 2002·Journal of Chromatography. a·A Kuksis

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