PMID: 45545Jan 1, 1977

Automatic measurement of the oxygen affinity of Cancer magister hemocyanin

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
H WajcmanH S Mason

Abstract

1. The P50 of Cancer Magister hemocyanin in hemolymph was 40.0 mm at 25 degrees C, nmax = 3.9; at 14 degrees C, 15.0 mm and 3.4; and at 10 degrees C. 10.2 mm and 3.3. 2. In the absence of Ca2+ and Mg2+, the P50 at 25 degrees C was in the range of 150 to 250 mm, depending upon pH. 3. Our results show that temperature variation and pH variation may be important factors in the physiological regulation of oxygen delivery in this species. 4. The automatic recording device of Imai et al. (1970) previously used for the study of hemoglobins, was found to be eminently suitable for the study of O2 affinities of hemocyanins.

References

Oct 7, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Maria T SannaMariagiuseppina Pellegrini

Citations

Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A J Uiterkamp, H S Mason
Apr 2, 1973·Biochemical and Biophysical Research Communications·J S Loehr, H S Mason
Jun 19, 1973·Biochemistry·T H MossH S Mason
Apr 9, 1974·Biochemistry·K Miller, K E Van Holde
Jul 1, 1959·Archives of Biochemistry and Biophysics·L C THOMSONH S Mason
Sep 1, 1964·Comparative Biochemistry and Physiology·J L LARIMER, A F RIGGS

Related Concepts

Dioxygen
Calcium
Hemolymph
Neoplasms
Oxygen Affinity
Uca
Metacarcinus magister
Hemocyanin Activity
Hemocyanin
Hemoglobin

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