PMID: 3753777Jan 10, 1986Paper

Automatic reading of DNA sequencing gel autoradiographs using a large format digital scanner

Nucleic Acids Research
J K ElderE M Southern

Abstract

We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DNA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band position and intensity. The sequence read in this way is as accurate as that read by an expert.

References

Jan 11, 1984·Nucleic Acids Research·M Komaromy, H Govan
Jan 11, 1984·Nucleic Acids Research·R Staden
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong

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Citations

Feb 1, 1994·Electrophoresis·C A Glasbey, F Wright
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·B Arveiler, D J Porteous
Jan 1, 1992·Bio Systems·Y TakefujiA Zaritsky
Jan 1, 1991·Mutation Research·J LindseyH J Cooke
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R TizardI Bronstein
Mar 11, 1988·Nucleic Acids Research·J West
Apr 11, 1988·Nucleic Acids Research·S StåhlM Uhlén
Jun 26, 1989·Nucleic Acids Research·R C AllshireN D Hastie
Mar 25, 1989·Nucleic Acids Research·P Richterich
Jun 25, 1991·Nucleic Acids Research·M Chee
Sep 25, 1993·Nucleic Acids Research·M C GiddingsL M Smith
May 17, 2005·Nucleic Acids Research·Troy Zerr, Steven Henikoff
Nov 1, 1987·Genomics·L E HoodL M Smith
Jun 1, 1994·IEEE Transactions on Bio-medical Engineering·J T IvesT G Stockham
Apr 1, 1989·Electrophoresis·W EhrhardtV Neuhoff
Feb 1, 1991·Analytical Biochemistry·D R SiemieniakJ L Slightom
Mar 1, 1990·Analytical Chemistry·G L Trainor
Oct 1, 1991·Journal of Electrocardiology·L E Widman, L S Hines
Dec 7, 1988·Journal of Theoretical Biology·W Bains, G C Smith
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·A T BankierE Preddie
Oct 4, 1991·Science·T HunkapillerL Hood
Oct 14, 1988·Science·U LandegrenL Hood

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