Automating the application of smart materials for protein crystallization

Acta Crystallographica. Section D, Biological Crystallography
Sahir KhurshidNaomi E Chayen

Abstract

The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as `smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.

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Citations

Jun 24, 2017·Protein Science : a Publication of the Protein Society·Raghurama P HegdeUdupi A Ramagopal
Apr 6, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·T ShilpaAbdul Ajees Abdul Salam
Aug 10, 2019·Journal of Applied Crystallography·Sylvain EngilbergeEric Girard
Oct 3, 2017·Crystal Growth & Design·Kyle A NordquistAndrew H Bond

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Methods Mentioned

BETA
X-ray
atomic force microscopy

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