Automation of Harboe method for the measurement of plasma free hemoglobin.

Journal of Clinical Laboratory Analysis
Hee-Jung ChungDo Hoon Lee

Abstract

Although plasma free hemoglobin (fHb) test is important for assessing intravascular hemolysis, it is still dependent on the gold standard Harboe method using manual and labor-intensive spectrometric measurements at the wavelength of 380-415-450 nm. We established an automated fHb assay using a routine chemistry autoanalyzer that can be tuned to a wavelength of 380-416-450 nm. The linearity, precision, accuracy, correlation, and sample carryover of fHb measurement using TBA200FRneo method and manual Harboe method were evaluated, respectively. fHb values measured by manual Harboe method were compared with those measured by our new automated TBA200FRneo method. fHb measurements were linear in the range of 0.05~38.75 µmol/L by TBA200FRneo and 0.05~9.69 µmol/L by manual Harboe method. Imprecision analysis (%CV) revealed 0.9~2.8% for TBA200FRneo method and 5.3~13.6% for the manual Harboe method. Comparison analysis showed 0.9986 of correlation coefficient (TBA200FRneo = 0.970 × Harboe + 0.12). In analytical accuracy analysis, the manual Harboe method revealed about 4 times higher average total error % (12.2%) than the TBA200FRneo automated method (2.8%). The sample carryover was -0.0016% in TBA200FRneo method and 0.0038% in Harboe me...Continue Reading

References

Mar 1, 1986·American Journal of Clinical Pathology·F G SoloniK Amazon
Jun 14, 2003·The British Journal of Mathematical and Statistical Psychology·Robert A Cribbie, H J Keselman
Jan 1, 1959·Scandinavian Journal of Clinical and Laboratory Investigation·M HARBOE
Apr 7, 2005·JAMA : the Journal of the American Medical Association·Russell P RotherMark T Gladwin
Feb 16, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Moon-Woo SeongDo-Hoon Lee
Jan 1, 1984·The Journal of Automatic Chemistry·P M Broughton
Jan 1, 2009·Indian Journal of Clinical Biochemistry : IJCB·Madhur M Goyal, Anjan Basak
Jul 16, 2015·Frontiers in Physiology·Ann Smith, Russell J McCulloh
Sep 15, 2018·Annals of Laboratory Medicine·Matthijs Oyaert, Joris Delanghe

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