Automation of MLST using third-generation liquid-handling technology.

Molecular Biotechnology
C B SullivanS C Clarke

Abstract

The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae, and Staphylococcus aureus. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting. Here we describe the automation of MLST on a third-generation liquid-handling robot.

Citations

Jul 8, 2010·Antimicrobial Agents and Chemotherapy·Muhamed-Kheir TahaIzabela Wasko
May 23, 2007·Antimicrobial Agents and Chemotherapy·Muhamed-Kheir TahaMaria Leticia Zarantonelli
Jul 27, 2010·Canadian Journal of Microbiology·José Júlio Costa SidrimMarcos Fábio Gadelha Rocha
Dec 15, 2010·International Journal of Microbiology·Donald InverarityGiles Edwards
Nov 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A van BelkumUNKNOWN European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM)
Oct 1, 2020·Food Science and Biotechnology·Anshul SharmaYoung-Seo Park

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