Automation of nucleic acid extraction for NAT screening of individual blood units

Transfusion
D H Lee, A M Prince

Abstract

Automation of NAT for single units of blood is currently hampered by the labor-intensive steps involved in the extraction of nucleic acids from samples before the amplification procedures. A new method has been developed for the automation of these steps using hydrophilic polyvinylidene fluoride (PVDF) filter plates. Quantitative nucleic acid recoveries from sera containing HCV, HIV, HBV, HAV, and human parvovirus B19 and from 3H-labeled HCV RNA were determined in parallel by the semi-automated PVDF method and a single-column method (Qiagen). Quantitative PCR was performed. Similar recoveries of HCV, HIV, and HBV (with silica beads) were observed with the PVDF method and with the Qiagen single-column method. The sensitivity of the PVDF-based PCR assay for HCV, HIV, and HBV in serially diluted serum samples was always within two serial dilutions of that obtained when the Qiagen single-column method was used in the same assays. With the use of 3H-labeled HCV RNA, recoveries of approximately 70 percent were found by both methods. The PVDF method will permit full automation of the simultaneous extraction of nucleic acid from sera containing HCV, HIV, and HBV. This procedure will permit NAT screening of individual units of blood, wi...Continue Reading

References

May 1, 1992·PCR Methods and Applications·A RavaggiE Cariani
Feb 1, 1992·The Pediatric Infectious Disease Journal·K K LeeL Fernando
Sep 1, 1991·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Sep 11, 1985·Nucleic Acids Research·E T Schenborn, R C Mierendorf
Jan 1, 1994·Vox Sanguinis·E Sandborg, M Thornton
Jan 1, 1994·Vox Sanguinis·J Vermylen, K Peerlinck
Apr 1, 1994·PCR Methods and Applications·J LaiL Andrus
Jun 27, 1996·The New England Journal of Medicine·G B SchreiberJ J Korelitz
Jan 26, 1999·Ophthalmology·C P Wilkinson
Jan 29, 1999·Journal of Virological Methods·K FransenG van der Groen

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Citations

Dec 3, 2003·Transfusion·Ronald O Gilcher
Jan 19, 2006·Clinical Microbiology Reviews·Omana V NainanHarold S Margolis
Jul 1, 2005·Transfusion·Lutz PichlVolkmar Schottstedt
Jul 1, 2005·Parasite Immunology·A FaridG T Strickland
Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jin-Won YounYoung Chul Sung
Jun 13, 2006·Journal of Medical Primatology·Mohamed Tarek M ShataAlfred M Prince
Sep 1, 2004·International Journal of Cardiology·Paolo Rebulla, Rosaria Giordano
Feb 28, 2002·Archives of Pathology & Laboratory Medicine·Frederick L Kiechle, Xinbo Zhang

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