Automation of solid-phase microextraction in high-throughput format and applications to drug analysis

Analytical Chemistry
Dajana VuckovicJ Pawliszyn

Abstract

The automation of solid-phase microextraction (SPME) coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) was accomplished using a 96 multiwell plate format, a SPME multifiber device, two orbital shakers, and a three-arm robotic system. Extensive optimization of the proposed setup was performed including coating selection, optimization of the fiber coating procedure, confirmation of uniform agitation in all wells, and the selection of the optimal calibration method. The system allows the use of pre-equilibrium extraction times with no deterioration in method precision due to reproducible timing of extraction and desorption steps and reproducible positioning of all fibers within the wells. The applicability of the system for the extraction of several common drugs is demonstrated. The optimized multifiber SPME-LC-MS/MS was subsequently fully validated for the high-throughput analysis of diazepam, lorazepam, nordiazepam, and oxazepam in human whole blood. The proposed method allowed the automated sample preparation of 96 samples in 100 min, which represents the highest throughput of any SPME technique to date, while achieving excellent accuracy (87-113%), precision (<or=20% RSD), and sensitivity (limit of quantitat...Continue Reading

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Citations

Oct 7, 2008·Analytical and Bioanalytical Chemistry·Sanja RisticevicJanusz Pawliszyn
Sep 4, 2009·Analytical and Bioanalytical Chemistry·Hiroyuki Kataoka
Nov 3, 2011·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Marcin WąsowiczStuart McCluskey
Nov 13, 2013·Analytica Chimica Acta·Fatemeh Mousavi, Janusz Pawliszyn
Feb 25, 2014·Analytica Chimica Acta·Jialiang PanGongke Li
Mar 12, 2010·Journal of the American Society for Mass Spectrometry·Jian PeiRobert T Kennedy
Jan 29, 2011·Chemical Reviews·Gangfeng OuyangJanusz Pawliszyn
Apr 5, 2012·Journal of Analytical Toxicology·David AndersenKristian Linnet
Sep 13, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroyuki Kataoka
Sep 9, 2011·Bioanalysis·Victoria SamanidouKonstantinos Rentifis
Dec 3, 2014·Journal of Chromatography. a·Nathaly Reyes-GarcésJanusz Pawliszyn
May 23, 2012·Bioanalysis·Christopher Singleton
Dec 18, 2013·Analytical and Bioanalytical Chemistry·Ezel BoyacıJanusz Pawliszyn
Apr 18, 2013·Bioanalysis·Akira Namera, Takeshi Saito
Jan 5, 2011·Journal of Pharmaceutical and Biomedical Analysis·Hiroyuki Kataoka, Keita Saito
Sep 6, 2008·Journal of Pharmaceutical and Biomedical Analysis·Erasmus Cudjoe, Janusz Pawliszyn
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Nov 19, 2014·Analytical Chemistry·Afsoon Pajand BirjandiJanusz Pawliszyn
Apr 22, 2011·Angewandte Chemie·Dajana VuckovicJanusz Pawliszyn
Jun 22, 2012·Se pu = Chinese journal of chromatography·Shengxiang Jiang, Juanjuan Feng

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