Autonomic regulation of pacemaker activity: role of heart nitric oxide synthases

American Journal of Physiology. Heart and Circulatory Physiology
Andrea Lorena FelletJ Bustamante

Abstract

In autonomic-blocked rats treated with NG-nitro-L-arginine methyl ester (L-NAME, 7.5 mg/kg), heart rate increased 18% and mean arterial pressure increased 48%. Thyroidectomy, along with autonomic blockade, hampered the chronotropic response but did not modify the effect on blood pressure. After 150 min of autonomic blockade, the experimental end point, total nitric oxide (NO) production by heart NO synthases (NOS) decreased 61%: from 54 to 21 nmol NO.min-1.g heart-1. Mitochondrial NOS (mtNOS) and sarcoplasmic reticulum endothelial NOS activities decreased 74% and 52%, respectively. Mitochondria isolated from whole heart showed a well-coupled oxidative phosphorylation with high respiratory control and ADP-to-O ratios, decreased mtNOS activity (55-60%), and decreased mtNOS protein expression (70%). Immunohistochemistry with anti-inducible NOS antibody linked to gold particles localized mtNOS at the inner mitochondrial membranes. Histochemical right atrial NOS (NADPH-diaphorase) decreased 55% after heart denervation. The effects of autonomic denervation on the NO system were partially prevented by thyroidectomy performed simultaneously with autonomic blockade. Western blot analysis indicated a very rapid mtNOS protein turnover (ha...Continue Reading

References

Aug 9, 1975·British Medical Journal·K E BrittonR P Ekins
Jun 1, 1992·Hypertension·A A PegoraroW H Beierwaltes
Jan 1, 1992·Neuroscience·S R Vincent, H Kimura
Mar 1, 1995·The American Journal of Physiology·J L BalligandT Michel
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L BalligandT Michel
Apr 15, 1994·The Journal of Physiology·X HanW R Giles
May 1, 1993·Circulation·R PolikarP Nicod
Jan 17, 1996·Biochemical and Biophysical Research Communications·T SekiM Naruse
Jun 6, 1998·The Journal of Biological Chemistry·A Tatoyan, C Giulivi
Jul 20, 1999·Journal of Applied Physiology·N HoganD J Paterson
Feb 15, 2001·The New England Journal of Medicine·I Klein, K Ojamaa
May 18, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S FrenchR S Balaban
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M C CarrerasJ J Poderoso
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A J KanaiJ Peterson
Aug 3, 2002·The Journal of Biological Chemistry·Sarah Liv ElferingCecilia Giulivi
Aug 8, 2002·Thyroid : Official Journal of the American Thyroid Association·W H Dillmann
Dec 17, 2002·Methods in Enzymology·Alberto BoverisAna Navarro
Apr 26, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A L FelletA M Balaszczuk
Jun 25, 2003·Hypertension·Isabel Rodríguez-GómezFélix Vargas
Sep 6, 2003·Circulation Research·P B MassionJ-L Balligand
May 5, 2004·Experimental Physiology·Andrea Lorena FelletAna Mar'a Balaszczuk
May 25, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ben J A JanssenThomas L Smith
Sep 30, 2004·Free Radical Biology & Medicine·Juanita BustamanteAlberto Boveris
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fernando AntunesEnrique Cadenas
Feb 8, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Gustavo F GonzalesAlberto Boveris
Feb 12, 2005·Journal of Applied Physiology·Tamara ZaobornyjAlberto Boveris
Apr 6, 2005·Trends in Pharmacological Sciences·Pedram Ghafourifar, Enrique Cadenas
Nov 18, 2005·Methods in Enzymology·Laura B ValdezAlberto Boveris

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Citations

Jan 21, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jae-Youn ChoiJung-Ho Cha
Mar 20, 2012·Metabolism: Clinical and Experimental·Lorena I SaratiAndrea L Fellet
Mar 28, 2009·Regulatory Peptides·W David KnightJ Michael Overton
Feb 14, 2015·Journal of Cellular and Molecular Medicine·Mücella KircaRainer Schulz
Jun 9, 2016·The Journal of Endocrinology·Natalia OgonowskiAndrea L Fellet
Oct 9, 2014·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Marie Dennis LeoDinesh Kumar
Oct 30, 2012·Developmental Biology·Carmine GentileChristopher J Drake
Feb 23, 2008·American Journal of Hypertension·Andrea L FelletAna M Balaszczuk
Oct 2, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Tamara Zaobornyj, Pedram Ghafourifar
Feb 24, 2015·Journal of Endocrinological Investigation·L RodríguezA Fellet
Apr 7, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Tamara ZaobornyjAlberto Boveris
Jan 15, 2013·International Journal of Molecular Medicine·Shunichi ShimizuYuji Kiuchi
Jun 19, 2021·Journal of Basic and Clinical Physiology and Pharmacology·Faten M A DiabEnas A Abdel-Hady

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