PMID: 8950295Jan 1, 1996Paper

Autonomous control of expression of genes for insulin-like growth factors during the proliferation and differentiation of C2C12 mouse myoblasts in serum-free culture

Life Sciences
Y YoshikoN Maeda

Abstract

The proliferation and differentiation of skeletal muscle cells in culture are usually controlled by serum components, and the differentiation can be induced by a reduction in the serum concentration. Insulin-like growth factors (IGFs) play a critical role in stimulating myoblast differentiation, and the expression of their genes is controlled by serum factors. We have found that C2C12 myoblasts are capable of proliferation and differentiation even in serum-free medium that does not contain peptide mitogens. During these processes in serum-free medium, the accumulation of mRNAs for IGFs in the cells was observed; and their levels increased with concomitant increases in creatine kinase activity and myotube formation and a decrease in DNA synthesis. Thus, the present results suggest that proliferation and differentiation of C2C12 cells are autonomously controlled and that the increase in the expression of the IGFs may be independent of exogenous components.

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Citations

Mar 13, 2003·Development, Growth & Differentiation·Akira YamaneHarold C Slavkin
Aug 7, 2003·Journal of Biomaterials Science. Polymer Edition·Anna Aihua LiPatricia L Chang
May 18, 2007·Biochemical and Biophysical Research Communications·Kazuki OmoteyamaMinoru Takagi
Jan 28, 2017·Thyroid : Official Journal of the American Thyroid Association·Tsubasa MurataShioko Kimura
Jul 17, 2010·Journal of Applied Physiology·Chau-Zen WangChia-Hsin Chen

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