Autonomous production of 1,4-butanediol via a de novo biosynthesis pathway in engineered Escherichia coli

Metabolic Engineering
Huaiwei Liu, Ting Lu

Abstract

1,4-Butanediol (BD) is an important chemical that is widely used in industry with an annual demand of one million metric tons. Here we report a modular development of engineered bacteria for successful BD bio-production. Using a systems engineering concept, we partitioned our development into two parts: namely BD biosynthesis and production control. The former was implemented through a de novo pathway that functions as an enzymatic reactor, while the latter was accomplished via synthetic circuits serving as genetic controllers. To facilitate development, the carbon utilizations were also partitioned into two routes. d-xylose was exclusively designated for BD production with other carbon sources utilized for cellular growth. Additionally, a quorum-sensing mechanism was exploited for production control, and the resulting strain was capable of autonomous production of BD. This study represents an example of the synergy between synthetic biology and metabolic engineering, affirming the need for deeper integration of the two fields.

References

Oct 23, 2003·Journal of the American Chemical Society·Wei NiuJ W Frost
Dec 19, 2006·Journal of Bacteriology·Craig StephensUrs Jenal
Nov 11, 2008·Current Opinion in Biotechnology·Sung Kuk LeeJay D Keasling
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kechun ZhangJames C Liao
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Jul 9, 2009·The Journal of Biological Chemistry·Ulrike JohnsenPeter Schönheit
Jan 21, 2010·Cell·William J Holtz, Jay D Keasling
Apr 7, 2010·Biotechnology Advances·E Celińska
Aug 31, 2010·Nature Biotechnology·Christopher S HenryRick L Stevens
Oct 12, 2010·Science·Parayil Kumaran AjikumarGregory Stephanopoulos
May 13, 2011·Trends in Biotechnology·Jeong Wook LeeSang Yup Lee
May 24, 2011·Nature Chemical Biology·Harry YimStephen Van Dien
May 31, 2011·Current Opinion in Biotechnology·Fuzhong ZhangJay D Keasling
Aug 9, 2011·Nature Biotechnology·Jens Nielsen, Jay D Keasling
Sep 3, 2011·Science·Octavio Mondragón-PalominoJeff Hasty
Sep 16, 2011·Bioresource Technology·Huaiwei LiuWook-Jin Chung
Dec 14, 2011·Metabolic Engineering·Karen K CarterWilliam E Bentley
Dec 23, 2011·FEMS Yeast Research·Jens Nielsen, Jack T Pronk
Feb 9, 2012·Metabolic Engineering·Jay D Keasling
Apr 3, 2012·Journal of Biological Engineering·Martin DragositsIlias Tagkopoulos
May 19, 2012·Nature Chemical Biology·Elizabeth A FelnagleJames C Liao
May 19, 2012·Nature Chemical Biology·Jeong Wook LeeSang Yup Lee
Aug 10, 2012·Applied Microbiology and Biotechnology·Mervi H ToivariMarilyn G Wiebe
Dec 13, 2012·Applied Microbiology and Biotechnology·Huaiwei LiuWook-Jin Chung
Dec 19, 2012·Metabolic Engineering·William Bacchus, Martin Fussenegger
Mar 16, 2013·Chemical Reviews·Christine A Rabinovitch-DeereShota Atsumi
May 10, 2013·ACS Synthetic Biology·Sang Yup Lee
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·S M Nayeemul BariShah M Faruque
Oct 1, 2013·BioMed Research International·Amit Kumar ChaudharyJae Kyung Sohng
Oct 18, 2013·Nature Communications·Howard H Chou, Jay D Keasling
Oct 22, 2013·Nature Biotechnology·Robert H DahlJay D Keasling
Apr 2, 2014·Nature Reviews. Microbiology·Chris J Paddon, Jay D Keasling
Apr 20, 2014·Nature Chemical Biology·Jens NielsenBernhard Palsson
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peng XuMattheos Koffas

❮ Previous
Next ❯

Citations

Feb 9, 2016·Nature Chemical Biology·Yi-Shu TaiKechun Zhang
Feb 26, 2016·Journal of Molecular Biology·Kevin C MaTimothy K Lu
Sep 8, 2015·Current Opinion in Biotechnology·Amin ZargarWilliam E Bentley
Sep 1, 2015·Current Opinion in Biotechnology·Hannah ChungSang Yup Lee
Oct 4, 2015·Current Opinion in Biotechnology·Jason T BoockKristala Lj Prather
May 2, 2016·Current Opinion in Biotechnology·Anthony BurgardHarry Yim
Nov 11, 2016·Journal of Industrial Microbiology & Biotechnology·Byung Eun MinGyoo Yeol Jung
Dec 9, 2017·Frontiers in Microbiology·Maria K McClintockKechun Zhang
Sep 29, 2017·Critical Reviews in Biotechnology·Thomas DecoeneMarjan De Mey
Jan 31, 2018·Journal of Industrial Microbiology & Biotechnology·Shuobo ShiHuimin Zhao
Feb 3, 2018·Applied Microbiology and Biotechnology·Rhudith B CabulongWook-Jin Chung
Nov 30, 2018·Molecular Systems Biology·Felix MoserChristopher A Voigt
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Christina V Dinh, Kristala L J Prather
Jul 14, 2018·Applied Microbiology and Biotechnology·Kris Niño G ValdehuesaWook-Jin Chung
Apr 8, 2020·Frontiers in Microbiology·Wing-Jin LiNick Wierckx
May 18, 2020·Nature Communications·Gita Naseri, Mattheos A G Koffas
Jan 5, 2020·Applied Microbiology and Biotechnology·Angelo B BañaresWook-Jin Chung
Sep 15, 2020·Metabolic Engineering·Christopher J HartlineFuzhong Zhang
Feb 11, 2021·Critical Reviews in Biotechnology·Angelo B BañaresWook-Jin Chung
Mar 13, 2020·Biotechnology Journal·Yu Jung SohnSi Jae Park
Jul 1, 2018·International Journal of Biological Macromolecules·Charles C LeeKurt Wagschal
Nov 25, 2016·Current Opinion in Biotechnology·Julia I Martien, Daniel Amador-Noguez
Oct 24, 2017·Current Opinion in Chemical Biology·Sue Zanne Tan, Kristala Lj Prather
Aug 1, 2018·Analytical Chemistry·Mst Afroza KhatunYan Feng
Sep 4, 2021·Journal of Agricultural and Food Chemistry·Jie ChengLinggang Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

Bipolar Disorder

Bipolar disorder is characterized by manic and/or depressive episodes and associated with uncommon shifts in mood, activity levels, and energy. Discover the latest research this illness here.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Kazuhiro Tateda
Applied Microbiology and Biotechnology
H PriefertA Steinbüchel
Advances in Biochemical Engineering/biotechnology
Uwe T Bornscheuer
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Li HanYanhe Ma
© 2021 Meta ULC. All rights reserved