Autonomous regulation of proliferation and growth arrest in mouse primordial germ cells studied by mixed and clonal cultures

Experimental Cell Research
Y OhkuboN Nakatsuji

Abstract

In culture, mouse primordial germ cells (PGCs) proliferate and undergo growth arrest with a time course similar to that in vivo. It is unclear whether this behavior is regulated autonomously or by coexisting somatic cells. We performed mixed culture experiments using PGCs from 8.5- and 11.5-d.p.c. embryos and found no interaction between the PGCs and somatic cells at the two stages. Next, we carried out clonal culture of PGCs and examined the proliferation of and morphological change in individual clones. Such clonal culture did not reveal any subpopulation of PGCs with an increased growth rate or less differentiated characteristics, which might have been suggested by formation of the embryonic germ cell lines. Our results suggest that there is an autonomous regulation of growth and cell shape change in PGCs which occur as stochastical events but are not strictly timed by the number of cell divisions.

Citations

Mar 11, 2004·Development, Growth & Differentiation·Yasuhiro SakaiShohei Yamashina
Jul 17, 2008·The Journal of Reproduction and Development·Ryo KakegawaYoshihiko Hosoi
Apr 17, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Josephine Bowles, Peter Koopman
Jul 6, 2004·Molecular Reproduction and Development·Monica Bullejos, Peter Koopman
Aug 27, 2010·Annals of the New York Academy of Sciences·Caleb E Finch, Donna J Holmes
Jul 1, 1997·Developmental Biology·A McLaren, D Southee
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Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·E Rajpert-De MeytsN E Skakkebaek
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Jul 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Morgane Le RolleAnne-Amandine Chassot
Aug 24, 2007·Development·Josephine Bowles, Peter Koopman

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