Autophagic Induction Greatly Enhances Leishmania major Intracellular Survival Compared to Leishmania amazonensis in CBA/j-Infected Macrophages

Frontiers in Microbiology
Beatriz R S DiasPatrícia S T Veras

Abstract

CBA mouse macrophages control Leishmania major infection yet are permissive to Leishmania amazonensis. Few studies have been conducted to assess the role played by autophagy in Leishmania infection. Therefore, we assessed whether the autophagic response of infected macrophages may account for the differential behavior of these two parasite strains. After 24 h of infection, the LC3-II/Act ratio increased in both L. amazonensis- and L. major-infected macrophages compared to uninfected controls, but less than in chloroquine-treated cells. This suggests that L. amazonensis and L. major activate autophagy in infected macrophages, without altering the autophagic flux. Furthermore, L. major-infected cells exhibited higher percentages of DQ-BSA-labeled parasitophorous vacuoles (50%) than those infected by L. amazonensis (25%). However, L. major- and L. amazonensis-induced parasitophorous vacuoles accumulated LysoTracker similarly, indicating that the acidity in both compartment was equivalent. At as early as 30 min, endogenous LC3 was recruited to both L. amazonensis- and L. major-induced parasitophorous vacuoles, while after 24 h a greater percentage of LC3 positive vacuoles was observed in L. amazonensis-infected cells (42.36%) compa...Continue Reading

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Citations

Nov 19, 2019·Frontiers in Immunology·Patricia Sampaio Tavares VerasBeatriz Rocha Simões Dias
Jan 31, 2020·Frontiers in Cellular and Infection Microbiology·Aislan de Carvalho Vivarini, Ulisses Gazos Lopes
May 25, 2020·Parasitology Research·Francesca De FalcoSante Roperto
Jan 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Durgesh Manohar PitaleChandrima Shaha
Jan 8, 2020·Cells·Stephanie Maia AcuñaSandra Marcia Muxel

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Methods Mentioned

BETA
lipidation
genetic modification
electrophoresis
PCA

Software Mentioned

GraphPad Prism
SAS
Image Quant Las 4000
Image J
JMP

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