Autophagy diminishes the early interferon-β response to influenza A virus resulting in differential expression of interferon-stimulated genes

Cell Death & Disease
Brieuc P PerotMatthew L Albert

Abstract

Influenza A virus (IAV) infection perturbs metabolic pathways such as autophagy, a stress-induced catabolic pathway that crosstalks with cellular inflammatory responses. However, the impact of autophagy perturbation on IAV gene expression or host cell responses remains disputed. Discrepant results may be a reflection of in vivo studies using cell-specific autophagy-related (Atg) gene-deficient mouse strains, which do not delineate modification of developmental programmes from more proximal effects on inflammatory response. In vitro experiments can be confounded by gene expression divergence in wild-type cultivated cell lines, as compared to those experiencing long-term absence of autophagy. With the goal to investigate cellular processes within cells that are competent or incompetent for autophagy, we generated a novel experimental cell line in which autophagy can be restored by ATG5 protein stabilization in an otherwise Atg5-deficient background. We confirmed that IAV induced autophagosome formation and p62 accumulation in infected cells and demonstrated that perturbation of autophagy did not impact viral infection or replication in ATG5-stablized cells. Notably, the induction of interferon-stimulated genes (ISGs) by IAV was d...Continue Reading

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Citations

Mar 13, 2019·Cells·Mariana MarquesDaniela Ribeiro
Feb 29, 2020·FEMS Microbiology Letters·Jing ZhangZhijun Jie
Jun 3, 2020·Journal of Cell Science·Huildore Bommanna RanjithaSuresh H Basagoudanavar
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Dec 25, 2019·Cold Spring Harbor Perspectives in Medicine·Stephan LudwigYvonne Boergeling
Dec 31, 2021·Virulence·Ao ZhouBin Tang

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Methods Mentioned

BETA
chemical treatments
flow cytometry
ELISA
PCR
ELISAs

Software Mentioned

GSEA
Incucyte ZOOM System
geNorm
StepOnePlus
Ideas
Nanostring
R ( CRAN )
nSolver
ImageJ
nCounter

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