Abstract
Several studies have revealed the functional importance of autophagy in both adipogenesis and carcinogenesis. Here, we investigated autophagy as a link between tumorigenesis and adipogenesis using 3T3-L1 cells, which have been shown to closely mimic the in vivo differentiation process. The relative levels of LC3-II/I showed that autophagy was the highest after 4-6 days of initiation of differentiation and it diminished thereafter. Furthermore, chloroquine (CQ), a late autophagy inhibitor, effectively inhibited adipogenic differentiation of 3T3-L1 cells, suggesting that autophagy may have a positive impact on adipogenic differentiation. Notably, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that CQ completely blocked the mRNA expression of three adipokines (adiponectin, leptin, and peroxisome proliferator-activated receptor-γ (PPARγ)), which increased proportionally to adipocyte differentiation. Using adipokine antibody arrays, we also found that among 38 adipokines examined, 6 adipokines were significantly differentially regulated in mature adipocytes compared to those in preadipocytes. A comparative analysis of adipokine production revealed that CQ-treated adipocytes displayed...Continue Reading
References
Mar 20, 2003·Molecular and Cellular Endocrinology·Dennis NormanAndrea Fabbri
Sep 26, 2003·Oncogene·Puneeth IyengarPhilipp E Scherer
Jun 18, 2005·The Proceedings of the Nutrition Society·Hans Hauner
Sep 8, 2005·American Journal of Physiology. Endocrinology and Metabolism·Xiubin LiangLi-Jun Ma
Jul 18, 2006·Cancer Cell·Kurt DegenhardtEileen White
Jun 16, 2009·Cell·Robin MathewEileen White
Oct 22, 2009·Autophagy·Rebecca BaergaShengkan Jin
Feb 4, 2010·The Journal of Biological Chemistry·You-Tong WuHan-Ming Shen
Feb 1, 2011·Annals of the New York Academy of Sciences·Yingfeng Deng, Philipp E Scherer
Dec 14, 2011·Biochemical and Biophysical Research Communications·Takeshi YoshizakiHiroshi Maegawa
Mar 28, 2012·Hormones : International Journal of Endocrinology and Metabolism·Adeeb ShehzadYoung Sup Lee
Sep 18, 2012·Journal of the National Cancer Institute·Hua FangYves A DeClerck
Nov 3, 2012·Endocrinology·H J JansenR Stienstra
Jan 5, 2013·Cancer Research·Tomonori KimuraYoshitaka Isaka
Jan 15, 2013·Differentiation; Research in Biological Diversity·Stefan LechnerWerner Zwerschke
Jun 27, 2013·American Journal of Physiology. Endocrinology and Metabolism·Chongben ZhangZhen Yan
Sep 28, 2013·Journal of Obesity·Giovanni De Pergola, Franco Silvestris
Jul 22, 2014·Differentiation; Research in Biological Diversity·Vojtech SkopLudmila Kazdova
Jan 6, 2016·Scientific Reports·Jung Ok LeeHyeon Soo Kim
Nov 2, 2016·Journal of Cellular Physiology·Hye Kyoung SungGary Sweeney
Jan 6, 2017·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Jae-Hyeon KimMichael Lee
Mar 16, 2017·Journal of Cellular Physiology·Sung-Hee HwangMichael Lee
Apr 21, 2017·Carcinogenesis·Harrison M PenroseSuzana D Savkovic
Jul 29, 2017·Nature Reviews. Cancer·Jean M Mulcahy LevyAndrew Thorburn
Nov 2, 2017·Oncotarget·Yu GuiLi Chen
Mar 30, 2018·Endocrine-related Cancer·Stefano MangiolaNiall M Corcoran
Jun 27, 2018·Autophagy·Mario MautheFulvio Reggiori