Autophagy Is Independent of the Chondroprotection Induced by Platelet-Rich Plasma Releasate

BioMed Research International
Fan YangChangqing Zhang

Abstract

Platelet-rich plasma (PRP) has been shown to be a promising therapeutic agent against osteoarthritis (OA), whereas its chondroprotection mechanism is not fully elucidated. Autophagy is considered an important biological process throughout the development of OA. Therefore, the objective of the present study is to investigate the role of autophagy in the chondroprotection and compare the effects of releasate between L-PRP and P-PRP. PRP were prepared from rat blood. Rat chondrocytes pretreated in the presence or absence of interleukin-1 beta (IL-1β) were incubated with PRP releasate. The expressions of OA-related genes and autophagy-related genes were determined by RT-PCR and western blot, respectively. Autophagic bodies were assessed by transmission electron microscopy and the autophagy flux was monitored under the confocal microscopy. The effect of PRP on autophagy was further investigated in the milieu of autophagy activator, rapamycin, or autophagy inhibition by downregulation of Atg5. The effect of PRP on cartilage repair and autophagy was also evaluated in an OA rat model. In vitro, PRP releasate increased the expression of the anabolic genes, COL2 and Aggrecan, and decreased the expression of the catabolic genes, whereas t...Continue Reading

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Citations

Jul 5, 2019·Current Pain and Headache Reports·Ivan UritsVwaire J Orhurhu
Oct 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·David ScullyAntonios Matsakas
Feb 19, 2020·Journal of Equine Veterinary Science·Livia Camargo Garbin, Christine S Olver

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Methods Mentioned

BETA
enzyme linked immunosorbent assay
transfection
PCR
flow cytometry

Software Mentioned

SPSS

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