Autophagy is involved in ethanol-induced cardia bifida during chick cardiogenesis

Cell Cycle
Shuai LiX Yang

Abstract

Excess alcohol consumption during pregnancy has been acknowledged to increase the incidence of congenital disorders, especially the cardiovascular system. However, the mechanism involved in ethanol-induced cardiac malformation in prenatal fetus is still unknown. We demonstrated that ethanol exposure during gastrulation in the chick embryo increased the incidence of cardia bifida. Previously, we reported that autophagy was involved in heart tube formation. In this context, we demonstrated that ethanol exposure increased ATG7 and LC3 expression. mTOR was found to be inhibited by ethanol exposure. We activated autophagy using exogenous rapamycin (RAPA) and observed that it induced cardiac bifida and increased GATA5 expression. RAPA beads implantation experiments revealed that RAPA restricted ventricular myosin heavy chain (VMHC) expression. In vitro explant cultures of anterior primitive streak demonstrated that both ethanol and RAPA treatments could reduce cell differentiation and the spontaneous beating of cardiac precursor cells. In addition, the bead experiments showed that RAPA inhibited GATA5 expression during heart tube formation. Semiquantitative RT-PCR analysis indicated that BMP2 expression was increased while GATA4 expr...Continue Reading

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Citations

Oct 30, 2016·Journal of Agricultural and Food Chemistry·Lin-Rui GaoXuesong Yang
Sep 9, 2017·Journal of Cellular Physiology·Jing ZhangXuesong Yang

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Methods Mentioned

BETA
PCR
transmission electron microscopy
flow cytometry

Software Mentioned

Olympus
Image
FISH Olympus
CN4000
Quantity One
VideoMach
Pro Plus
SPSS

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