Autophagy is involved in high glucose-induced heart tube malformation

Cell Cycle
Guang WangXuesong Yang

Abstract

Both pre-gestational and gestational diabetes have an adverse impact on heart development, but little is known about the influence on the early stage of heart tube formation. Using early gastrulating chick embryos, we investigated the influence of high glucose on the process of heart tube formation, specifically during the primary heart field phase. We demonstrated that high-glucose exposure resulted in 3 types of heart tube malformation: 1) ventricular hypertrophy, 2) ventricular hypertrophy with dextrocardia and 3) ventricular hypertrophy and dextrocardia with the fusion anomaly of a bilateral primary heart tube. Next, we found that these malformation phenotypes of heart tubes might mainly originate from the migratory anomaly of gastrulating precardiac mesoderm cells rather than cell proliferation in the developmental process of bilateral primary heart field primordia. The treatment of rapamycin (RAPA), an autophagy inducer, led to a similar heart tube malformation phenotype as high glucose. Additionally, high-glucose exposure promoted the expression of the key autophagy protein LC3B in early chick tissue. Atg7 is strongly expressed in the fusion site of bilateral primary heart tubes. All of these data imply that autophagy co...Continue Reading

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Citations

Jun 17, 2016·Expert Review of Cardiovascular Therapy·Jaime A RiquelmeSergio Lavandero
Sep 9, 2017·Journal of Cellular Physiology·Jing ZhangXuesong Yang
Jan 13, 2018·Biology Letters·Wei Xie, Jun Zhou
Apr 21, 2020·Journal of the American Heart Association·Emmi Helle, James R Priest
Sep 9, 2015·The Journal of Experimental Biology·Guang WangXuesong Yang

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Methods Mentioned

BETA
PCR
Fluorescence
GTPase
xenograft

Software Mentioned

VideoMach
FISH Olympus
CN4000
Image
Olympus
Pro Plus
SYNGENE
SPSS

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