Autophagy is required for stem cell mobilization by G-CSF.

Blood
Lucie Leveque-El MouttieSteven W Lane

Abstract

Granulocyte colony-stimulating factor (G-CSF) is widely used clinically to prevent neutropenia after cytotoxic chemotherapy and to mobilize hematopoietic stem cells (HSCs) for transplantation. Autophagy, a process of cytoplasmic component recycling, maintains cellular homeostasis and protects the cell during periods of metabolic stress or nutrient deprivation. We have observed that G-CSF activates autophagy in neutrophils and HSCs from both mouse and human donors. Furthermore, G-CSF-induced neutrophil and HSC mobilization is impaired in the absence of autophagy. In contrast, autophagy is dispensable for direct HSC mobilization in response to the CXCR4 antagonist AMD3100. Altogether, these data demonstrate an important role for G-CSF in invoking autophagy within hematopoietic and myeloid cells and suggest that this pathway is critical for ensuring cell survival in response to clinically relevant cytokine-induced stress. These findings have direct relevance to HSC transplantation and the increasing clinical use of agents that modulate autophagy.

References

Mar 28, 1998·The Journal of Biological Chemistry·T Noda, Y Ohsumi
Apr 20, 2005·The Journal of Experimental Medicine·Hal E BroxmeyerEdward F Srour
May 12, 2006·Seminars in Cell & Developmental Biology·Gary B Willars
Feb 26, 2008·Cell·Stuart H Orkin, Leonard I Zon
Mar 14, 2008·The EMBO Journal·Mónica A DelgadoVojo Deretic
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Feb 23, 2011·The Journal of Experimental Medicine·Monika MortensenAnna Katharina Simon
Sep 6, 2011·Cytokine·James Harris
Sep 15, 2012·Annals of the Rheumatic Diseases·Neng-Yu LinJörg H W Distler
Feb 8, 2013·Nature·Matthew R WarrEmmanuelle Passegué

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Citations

Dec 15, 2015·International Journal of Hematology·Zhao Wang, Hideo Ema
Aug 24, 2016·The Journal of Experimental Medicine·Besnik BajramiHongbo R Luo
Jan 7, 2017·Autophagy·Federico PietrocolaGuido Kroemer
Feb 28, 2017·Nature·Theodore T HoEmmanuelle Passegué
Aug 15, 2017·Autophagy·Thomas RiffelmacherAnna Katharina Simon
Jun 23, 2018·International Journal of Radiation Biology·Melis KarabulutogluChristophe Badie
Sep 21, 2020·Aging Cell·Yixuan FangJianrong Wang
Mar 7, 2019·Current Protocols in Immunology·Kate A Markey, Kate H Gartlan
Dec 12, 2018·Nature Reviews. Immunology·Alexander J Clarke, Anna Katharina Simon
Apr 29, 2020·Burns and Trauma·Yao Yu, Bingwei Sun
Oct 30, 2020·Metabolites·Wesley H Godfrey, Michael D Kornberg
Nov 19, 2020·Cellular & Molecular Immunology·Cristina Tecchio, Marco Antonio Cassatella
Nov 9, 2021·Molecular and Cellular Biology·Shun-Suke SakaiMasaaki Komatsu

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