Autophagy plays a dual role during intracellular siRNA delivery by lipoplex and polyplex nanoparticles

Acta Biomaterialia
Wen SongChuanxu Yang

Abstract

Growing evidence indicates that autophagy plays a vital role during intracellular DNA delivery mediated by lipoplex and polyplex nanoparticles. However, autophagy in intracellular siRNA delivery has not been well understood. In this study, lipofectamine 2000 and chitosan were used to formulate lipoplex and polyplex with siRNA for systematically investigating the interplay between siRNA delivery and autophagy. After transfection of H1299 cells with lipoplex and polyplex, the number of autophagic vacuoles was increased significantly indicated by the accumulation of monodansylcadaverine (MDC) staining. Western blot revealed that the LC3-II expression was significantly increased after transfection, whereas p-mTOR expression was not influenced apparently. In addition, small-molecule autophagy modulators significantly affected transfection efficiency. Specifically, the mTOR-dependent autophagy inducer rapamycin enhanced the knockdown efficiency of both lipoplex and polyplex, whereas mTOR-dependent autophagy inhibitor 3-methyladenine (3-MA) suppressed their silencing efficiency. On the contrary, mTOR-independent autophagy inducer LiBr decreased whereas mTOR-independent autophagy inhibitor thapsigargin (TG) increased the knockdown effi...Continue Reading

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Citations

Nov 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tanja BusUlrich S Schubert
Sep 29, 2019·Biomolecules·Shima TavakolAlan Prem Kumar
May 3, 2020·Current Stem Cell Research & Therapy·Yang Gao, Tao Zhang

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