Autophagy Protects From Uremic Vascular Media Calcification.

Frontiers in Immunology
Bianca FrauscherPhilipp Eller

Abstract

Chronic kidney disease and diabetes mellitus are associated with extensive media calcification, which leads to increased cardiovascular morbidity and mortality. Here, we investigated the role of autophagy in the pathogenesis of uremic vascular media calcification. DBA/2 mice were fed with high-phosphate diet (HPD) in order to cause vascular calcification. DBA/2 mice on standard chow diet were used as control. In parallel, autophagy and its response to rapamycin, 3-methyladenine (3-MA), and bafilomycin were studied in an in vitro model using mouse vascular smooth muscle cells (MOVAS). DBA/2 mice on HPD developed severe vascular media calcification, which is mirrored in vitro by culturing MOVAS under calcifying conditions. Both, in vitro and in vivo, autophagy significantly increased in MOVAS under calcifying conditions and in aortas of HPD mice, respectively. Histologically, autophagy was located to the aortic Tunica media, but also vascular endothelial cells, and was found to continuously increase during HPD treatment. 3-MA as well as bafilomycin blocked autophagy in MOVAS and increased calcification. Vice versa, rapamycin treatment further increased autophagy and resulted in a significant decrease of vascular calcification in ...Continue Reading

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Citations

Mar 25, 2019·Journal of Cardiovascular Pharmacology and Therapeutics·Belay Tesfamariam
Apr 17, 2020·International Journal of Molecular Sciences·Sun Joo LeeJae-Han Jeon
Jul 9, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun-Seop SongJoseph Loscalzo
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Sep 29, 2020·Journal of Translational Internal Medicine·Pengbo WangYingxian Sun
Apr 3, 2019·International Journal of Molecular Sciences·Miguel CarracedoMagnus Bäck
Nov 6, 2020·Journal of Cellular and Molecular Medicine·Jing-Wei GaoPin-Ming Liu
Dec 2, 2020·International Journal of Molecular Sciences·Cédric H G NeutelPieter-Jan Guns
Oct 28, 2019·Pharmacology & Therapeutics·Kanchan PhadwalVicky E MacRae
Mar 30, 2021·Clinical Kidney Journal·Paola Ciceri, Mario Cozzolino
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Kanchan PhadwalVicky E MacRae
May 29, 2021·Frontiers in Cardiovascular Medicine·Yi LuZhejun Cai

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Methods Mentioned

BETA
FCS
PCR
Protein Assay
electrophoresis
Assay
chip

Software Mentioned

GraphPad
Image Lab
GraphPad Prism

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