Autophagy proteins play cytoprotective and cytocidal roles in leucine starvation-induced cell death in Saccharomyces cerevisiae.

Autophagy
Slawomir A Dziedzic, Allan B Caplan

Abstract

Autophagy is essential for prolonging yeast survival during nutrient deprivation; however, this report shows that some autophagy proteins may also be accelerating population death in those conditions. While leucine starvation caused YCA1-mediated apoptosis characterized by increased annexin V staining, nitrogen deprivation triggered necrotic death characterized by increased propidium iodide uptake. Although a Δatg8 strain died faster than its parental strain during nitrogen starvation, this mutant died slower than its parent during leucine starvation. Conversely, a Δatg11 strain died slower than its parent during nitrogen starvation, but faster during leucine starvation. Curiously, although GFP-Atg8 complemented the Δatg8 mutation, this protein made ATG8 cells more sensitive to nitrogen starvation, and less sensitive to leucine starvation. These results were difficult to explain if autophagy only extended life but could be an indication that a second form of autophagy could concurrently facilitate either apoptotic or necrotic cell death.

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Citations

Aug 11, 2012·Endocrinology·Baosheng ChenD Michael Nelson
Apr 7, 2016·Cellular and Molecular Life Sciences : CMLS·Claudio Falcone, Cristina Mazzoni
Nov 12, 2013·FEMS Yeast Research·Su-Ju Lin, Nicanor Austriaco
Jun 2, 2018·Oxidative Medicine and Cellular Longevity·Michael R YeamanKarin Thevissen

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