Autophagy upregulation and apoptosis downregulation in DAHP and triptolide treated cerebral ischemia

Mediators of Inflammation
Yang YangMarong Fang

Abstract

It has previously been demonstrated that ischemic stroke activates autophagy pathways; however, the mechanism remains unclear. The aim of this study is to further investigate the role that autophagy plays in cerebral ischemia. 2, 4-diamino-6-hydroxy-pyrimidine (DAHP), for its nitric oxide synthase (NOS) inhibiting neuroprotective effect, and triptolide (TP), for its anti-inflammatory property, were selected to administer pre middle cerebral artery occlusion (MCAO). The drugs were administered 12 hours prior to MCAO. Both magnetic resonance imaging (MRI) and 2, 3, 5-triphenyltetrazolium chloride (TTC) staining showed that the drugs reduce the area of infarction. Immunoblotting analysis revealed increases in Beclin-1 and myeloid cell leukelia-1(Mcl-1) in treated rats. This could be a contributing factor to the reduction in autophagy induced damage. Immunochemistry and western blot showed that mTOR expression in treated rats was marginally different 24 h after injury, and this could also be significant in the mechanism. Furthermore, terminal deoxynucleotidyl transferase- (TdT-) mediated dUTP nick end labeling (TUNEL) staining proved that the drugs are effective in reducing apoptosis. The upregulation of Beclin-1 and Mcl-1 and down...Continue Reading

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Citations

Nov 18, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shi BaiMarong Fang
Sep 8, 2015·Journal of Integrative Medicine·Xiao-ping HuangChang-qing Deng
Oct 11, 2016·The Journal of Nutritional Biochemistry·Yaqian ZhaoXumei Zhang
Nov 9, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chunhua LiuZhuo Yang
Apr 8, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Qing-Shan LiuRui Liu
Feb 3, 2018·Oxidative Medicine and Cellular Longevity·Congcong FangXiaoxing Xiong
Aug 31, 2016·Molecular Medicine Reports·Zhiyou CaiGuanghui Chen
Jan 30, 2019·Frontiers in Neuroscience·Yulin SunGuozheng Ying
Jul 19, 2018·Behavioural Neurology·Yanhua QinMarong Fang
Dec 18, 2020·International Journal of Molecular Sciences·Woosuk KimDae Won Kim
Mar 11, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Meng WangYuchuan Ding
May 20, 2021·Oxidative Medicine and Cellular Longevity·Hui XuMingfu Wang
Jun 5, 2021·Journal of Ethnopharmacology·Yeqing WangMeili Guo

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Image
Pro Plus
SPSS

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