Autophosphorylation activity and association with Src family kinase of Sky receptor tyrosine kinase

Biochemical and Biophysical Research Communications
J ToshimaK Mizuno

Abstract

"Sky" is a putative receptor tyrosine kinase predominantly expressed in the brain. Sky, like Axl/Ufo/Ark and c-Eyk, has an extracellular domain composed of two immunoglobulin-like domains and two fibronectin type III domains. Immunoblot analysis using an antibody raised against a C-terminal peptide of Sky identified a 98-kDa Sky protein in COS cells transfected with sky cDNA (COS/sky cells). A 98-kDa protein in the immunoprecipitates with anti-Sky antibody was autophosphorylated on tyrosine, by in vitro kinase reaction. When the lysates of COS/sky cells were immunoprecipitated with anti-Sky antibody and immunoblotted with an anti-phosphotyrosine antibody, a 60-kDa phosphotyrosine-containing protein, in addition to the tyrosine-phosphorylated Sky, was detected. Using the anti-Src antibody, which is reactive to Src, Fyn and Yes, we obtained evidence for an association between the Src family tyrosine kinase and the tyrosine-phosphorylated Sky receptor. These results suggest that the Src family kinase may play an important role in signal transduction of the Sky receptor.

Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shoutian ZhuXu Wu
May 1, 1997·Pathology·K E Crosier, P S Crosier
Aug 1, 2015·Molecular Biology of the Cell·Yuki MiyamotoJunji Yamauchi
Jan 28, 2016·International Journal of Cancer. Journal International Du Cancer·Johannes C van der MijnHenk M W Verheul
Oct 19, 2004·Oncogene·Paul A BromannSara A Courtneidge
Sep 29, 1995·The Journal of Biological Chemistry·K OhashiK Mizuno
Jul 31, 2014·The Journal of Biological Chemistry·Khanh-Quynh N NguyenRaymond B Birge
Nov 18, 2015·International Journal of Oncology·Yan DuanHan Kiat Ho
Feb 5, 2019·Experimental Biology and Medicine·Pei-Ling HsuShaw-Jenq Tsai
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A M HealyH W Farber
Dec 7, 2018·Cancers·Sherri K SmartDouglas K Graham
Nov 30, 2019·Cell Communication and Signaling : CCS·Tal Burstyn-Cohen, Avi Maimon
Feb 1, 1997·Seminars in Perinatology·L G IsraelsS P Saxena

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.