Abstract
Cultured mouse peritoneal macrophages were found to be relatively impermeable to streptomycin. Based on radioactivity measurements and radioautographic evidence, macrophages were impermeable to tritiated dihydrostreptomycin for periods up to 20 hr of incubation. Little or no intracellular streptomycin could be detected even when incubation was carried out in the presence of therapeutic blood levels of carrier dihydrostreptomycin. When the cultured mouse macrophages were allowed to phagocytize staphylococci, yeast cells, or polystyrene latex particles in the presence of tritiated streptomycin, the impermeability of the cells to the antibiotic was not affected. These observations suggested that the process of phagocytosis does not facilitate the intracellular accumulation of streptomycin, as seems to be the case for the fixed phagocytic cells of the liver.
References
Jun 11, 1966·Nature·B HolmesR A Good
Jan 21, 1967·Nature·E Oxman, P F Bonventre
Feb 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·V I OYAMA, H EAGLE
Aug 21, 1959·Science·H EAGLE
Jul 1, 1960·The Journal of Experimental Medicine·G B MACKANESS
Aug 1, 1960·The Journal of Experimental Medicine·C JENKIN, B BENACERRAF
Jun 1, 1964·Journal of General Microbiology·G KARLSBADL MONACO
Apr 1, 1965·The Journal of Infectious Diseases·J A MORELLO, E E BAKER
Sep 1, 1965·Analytical Chemistry·R TYE, J D ENGEL
Apr 21, 1962·Nature·A J SBARRAW A BARDAWIL
Citations
Feb 1, 1984·The Journal of Pharmacy and Pharmacology·M StevensonR M Richards
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·M Maurin, D Raoult
Jun 19, 2002·Antimicrobial Agents and Chemotherapy·Stéphane CarrynPaul M Tulkens
Jan 4, 2008·Antimicrobial Agents and Chemotherapy·Ofir MenasheSima Yaron
Feb 8, 2011·Nature Reviews. Microbiology·Guy E Thwaites, Vanya Gant
Sep 28, 2016·Antimicrobial Agents and Chemotherapy·Floriana CappielloMaria Luisa Mangoni
May 23, 2012·The Journal of Antimicrobial Chemotherapy·Edurne ImbuluzquetaMaría J Blanco-Prieto
Jan 10, 2018·Frontiers in Cellular and Infection Microbiology·Tiva T VanCleaveMatthew B Lawrenz
Dec 7, 2018·Nature Methods·Mark R CronanDavid M Tobin
Feb 15, 1978·Biochemical Pharmacology·P Tulkens, A Trouet
Jun 1, 1978·Antimicrobial Agents and Chemotherapy·P F Bonventre, G Gregoriandis
Apr 1, 1981·Infection and Immunity·J A Devenish, D A Schiemann
Apr 1, 1972·Infection and Immunity·D AdamW Marget
Jan 9, 2004·Infectious Disease Clinics of North America·Stéphane CarrynPaul M Tulkens
Jul 1, 1970·Infection and Immunity·P F Bonventre, J G Imhoff
Apr 14, 2018·Microbiology Spectrum·Daisy VanrompayPatrick Butaye