PMID: 558344Apr 1, 1977Paper

Autoradiographic method for detection of lactate dehydrogenase-elevating virus-infected cells in primary mouse macrophage cultures

Journal of Virology
S L TongP G Plagemann

Abstract

Peritoneal cells from starch-injected Swiss mice were propagated in plastic petri dishes and on cover slips in a mouse L-cell-conditioned medium for 12 to 24 h and then infected with various multiplicities of lactate dehydrogenase-elevating virus (LDV). Over 95% of the cells in these cultures phagocytosed latex particles and were, therefore, considered macrophages. Infected and mock infected macrophage cultures were supplemented with [3H]uridine at various times after infection and with actinomycin D 30 min before addition of the [3H]uridine. After 1 or 2 h of further incubation, plate cultures were analyzed for LDV-specific RNA, and cover slip cultures were investigated by autoradiography. Other cultures were labeled in the absence of actinomycin D, and the culture fluid was analyzed for labeled LDV. There was a good correlation between the production of LDV-specific RNA and LDV and the number of heavily labeled cells in these cultures. The labeled cells in these cultures. The labeled cells, therefore, were equated with productively infected cells. Only a maximum of about 20% of the macrophages, however, became heavily labeled regardless of the multiplicity of infection or the time, after infection, at which the cells were exp...Continue Reading

References

May 1, 1975·The Journal of Experimental Medicine·C C StewartC Adles
Jun 11, 1975·The Journal of Experimental Medicine·C BiancoS C Silverstein
Nov 1, 1972·Journal of Virology·M B Darnell, P G Plagemann
Apr 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·P G Plagemann, H E Swim
Jan 1, 1965·The Journal of Experimental Medicine·Z A COHN, B BENSON

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Citations

Jan 1, 1995·Springer Seminars in Immunopathology·P G PlagemannK S Faaberg
Jan 1, 1989·Critical Reviews in Microbiology·W A Cafruny

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