PMID: 2113410Apr 23, 1990Paper

Autoradiographic studies in animal models of hemi-parkinsonism reveal dopamine D2 but not D1 receptor supersensitivity. I. 6-OHDA lesions of ascending mesencephalic dopaminergic pathways in the rat

Brain Research
W C GrahamG N Woodruff

Abstract

The selective dopaminergic antagonist ligands [3H]SCH 23390 and [3H]sulpiride were used to reveal autoradiographically dopamine D1 and D2 receptors, respectively, in brain sections from rats which had received unilateral 6-hydroxydopamine (6-OHDA) injections destroying ascending nigrostriatal neurones. The binding of both ligands to striatal sections was first shown to be saturable, reversible and of high affinity and specificity [( 3H]SCH 23390: Bmax 2.16 pmol/mg protein, Kd 1.4 nM; [3H]sulpiride; Bmax 0.67 pmol/mg protein, Kd 10.7 nM). After unilateral stereotaxic 6-OHDA injections, rats rotated contralaterally when challenged with apomorphine (0.5 mg/kg), or specific D1 or D2 agonists, SKF 38393 (1.0-5.0 mg/kg) and LY 171555 (0.05-0.5 mg/kg), respectively. Loss of forebrain dopaminergic terminals was assessed autoradiographically using [3H]mazindol to label dopamine uptake sites. A loss of approximately 90-95% of uptake sites was reproducibly accompanied by an enhanced density of binding ipsilaterally for the D2 ligand, [3H]sulpiride, in all areas of the striatum, but most markedly in the lateral areas. An increase in the D2 binding site density was also seen in the ipsilateral nucleus accumbens and the olfactory tubercle. I...Continue Reading

References

Jan 11, 1979·Nature·J W Kebabian, D B Calne
Jul 31, 1985·European Journal of Pharmacology·R RaismanY Agid
Aug 15, 1985·European Journal of Pharmacology·C PimouleS Z Langer
Dec 1, 1986·The Journal of Pharmacy and Pharmacology·G J KilpatrickC D Marsden
Oct 27, 1987·European Journal of Pharmacology·W C Graham, A R Crossman
Dec 1, 1987·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·P SeemanW W Tourtellotte
Dec 16, 1985·Brain Research·J O RinneU K Rinne
Jan 1, 1985·Journal of Neural Transmission·M Guttman, P Seeman
Jul 31, 1986·European Journal of Pharmacology·M BuonamiciG Di Chiara
Sep 28, 1984·Neuroscience Letters·T R JastrowM E Gnegy
Dec 1, 1981·Neuropharmacology·S B FreedmanG N Woodruff
Dec 10, 1984·Brain Research·M Herrera-Marschitz, U Ungerstedt
Feb 21, 1983·Brain Research·O GershanikR C Duvoisin

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Citations

May 1, 1996·Movement Disorders : Official Journal of the Movement Disorder Society·L V MetmanT N Chase
Dec 10, 2009·Neurochemical Research·Ho-Joo LeeAbesh Kumar Bhattacharjee
Jan 1, 1996·Neuroscience and Biobehavioral Reviews·J N JoyceB S Neal-Beliveau
Jun 21, 1991·Brain Research. Developmental Brain Research·P A RaoJ N Joyce
Sep 22, 2001·Pharmacology, Biochemistry, and Behavior·P M Robinet, M T Bardo
Jan 1, 1991·Acta Neurologica Scandinavica. Supplementum·S M Aquilonius
Sep 14, 2000·Brain & Development·Y Saito, S Takashima
Jul 1, 1994·Progress in Neurobiology·J SirviöP J Riekkinen
Oct 16, 2015·Neurotoxicity Research·Aderbal S AguiarRui D Prediger
Jan 12, 2011·Neurobiology of Disease·E L LaneS B Dunnett
Dec 7, 2007·Neuroscience and Biobehavioral Reviews·R Brandies, S Yehuda
Nov 2, 2007·Basic & Clinical Pharmacology & Toxicology·Tiina M KääriäinenPekka T Männistö

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